小鼠和人N-acetylglucosamine激酶分子克隆和表征。
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伯杰Hinderlich年代,M,施瓦茨科普夫M, Effertz K,发音W
小鼠和人N-acetylglucosamine激酶分子克隆和表征。
欧元。2000 267年6月;(11):3301 - 8。
- PubMed ID
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10824116 (在PubMed]
- 文摘
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N-Acetylglucosamine是由内生glycoconjugates退化和退化的膳食glycoconjugates糖苷酶。它进入氨基糖代谢的通路N-acetylglucosamine激酶的作用。在这项研究中我们报告的隔离和表征cDNA克隆小鼠酶编码。1029个碱基对的开放阅读框编码343个氨基酸分子质量预测的37.3 kDa。推导的氨基酸序列包含的八肽序列的匹配来自老鼠N-acetylglucosamine激酶的胰蛋白酶的乳沟。重组小鼠酶在大肠杆菌BL21细胞功能表达,它显示N-acetylglucosamine激酶活性以及N-acetylmannosamine激酶活性。人类N-acetylglucosamine激酶的完整cDNA序列的核苷酸序列来自几个表达序列标签。1032个碱基对的开放阅读框编码344个氨基酸和蛋白质分子质量预测的37.4 kDa。相似性人类和小鼠N-acetylglucosamine激酶在核苷酸水平86.6%和91.6%的氨基酸水平。氨基酸序列的小鼠和人类N-acetylglucosamine激酶显示序列相似性与其他糖激酶,和所有五个序列图案需要绑定的ATP糖激酶。 Tissue distribution of murine N-acetylglucosamine kinase revealed an ubiquitous occurrence of the enzyme and a very high expression in testis. The size of the murine mRNA was 1.35 kb in all tissues investigated, with the exception of testis, where it was 1.45 kb mRNA of the murine enzyme was continuously expressed during mouse development. mRNA of the human enzyme was expressed in all investigated human tissues, as well as in cancer cell lines. In both the tissues and the cancer cell lines, the human mRNA was 1.35 kb in size.