共焦immunolocalization牛血清白蛋白、血清retinol-binding蛋白质,interphotoreceptor retinoid-binding蛋白质牛视网膜上。

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邓肯T,法里斯RN, Wiggert B

共焦immunolocalization牛血清白蛋白、血清retinol-binding蛋白质,interphotoreceptor retinoid-binding蛋白质牛视网膜上。

摩尔粘度2006年12月22日,12:1632-9。

PubMed ID

17200663 (在PubMed

]

文摘

目的:最近,它已经表明,视黄醇的运输以及间隙分离杆感光细胞需要一个细胞外因子。Interphotoreceptor retinoid-binding蛋白(IRBP)是一个组件的Interphotoreceptor矩阵(IPM)和绑定视觉周期类维生素a。血清白蛋白和血清retinol-binding蛋白质(sRBP),蛋白质能够绑定类维生素a,也被报道称IPM的组件。感兴趣的知道出现在IPM的组件能够绑定的视觉周期类维生素a,也促进视紫红质再生。本研究的目的是确定血清白蛋白的本地化,sRBP, IRBP牛视网膜使用免疫荧光分析。方法:新鲜牛的眼睛,从当地屠宰场,获得在去核后立即修复。组织部分(100 microm)孵化与牛血清白蛋白(BSA)主要抗体,sRBP, IRBP。部分被洗然后孵化4 h和4“6-diamidino-2-phenylindole (DAPI), Alexa萤石(R) 488只山羊antimouse, Alexa萤石568山羊antirabbit二级抗体。部分分析了利用激光扫描共焦显微镜配备Nomarski光学。西方的牛视网膜组织的免疫印迹分析和蛋白质标准进行使用主BSA抗体,sRBP, IRBP显示各自的抗原特异性。 RESULTS: Immunoblot analysis showed that monoclonal anti-BSA was highly specific for BSA detecting only a single band at about 67 kDa. Antihuman sRBP and antibovine IRBP were also highly specific, recognizing a single band at about 25 and about 133 kDa, respectively. No immunopositive bands were observed in bovine neural retinal when probed with the anti-sRBP antibody; however, a single immunoreactive band at about 67 and about 133 kDa was detected in bovine neural retina by the anti-BSA and IRBP antibodies, respectively. Immunofluorescence analysis showed labeling for IRBP throughout the IPM. IRBP labeling was especially associated with the outer segments of photoreceptors and also with the apical surface of the retinal pigment epithelium. Immunofluorescence labeling for serum albumin was associated only with the lumen of retinal and choroidal blood vessels. Staining for both serum albumin and sRBP in the IPM was negative. CONCLUSIONS: Immunofluorescence analysis of fresh bovine eyes using antibodies to BSA and sRBP clearly shows that serum albumin and sRBP are not components of bovine IPM. IRBP, on the other hand, is localized to the IPM where it is available for the binding and transport of visual cycle retinoids. From these data we conclude that serum albumin and sRBP are not factors that could participate in the binding as well as transport of visual cycle retinoids in the IPM of bovine retina.

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药物载体

药物	航空公司	类	生物	药理作用	行动
维他命A	Retinol-binding蛋白3	蛋白质	人类	未知的	粘结剂	细节