协会receptor-alpha雌激素和孕激素受体表达与荷尔蒙乳腺致癌作用:宿主微环境的作用。
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蒙特罗吉拉德G, Vanzulli SI、Cerliani JP Bottino MC, Bolado J,船帆座J, Becu-Villalobos D, F Benavides,为年代,Patel V, Molinolo, Lanari C
协会receptor-alpha雌激素和孕激素受体表达与荷尔蒙乳腺致癌作用:宿主微环境的作用。
乳腺癌研究》2007;9 (2):R22。
- PubMed ID
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17341305 (在PubMed]
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简介:甲孕酮(MPA)诱发雌激素受体(ER)阳性和孕激素受体(PR)阳性入侵乳腺导管癌在BALB / c小鼠。我们试图重现这个MPA C57BL / 6小鼠肿瘤模型,因为他们的基因工程中广泛使用。在这个实验设置中,我们研究了MPA的致癌的形态学变化所诱导的乳腺MPA和孕激素,和MPA-treated ER和PR的表达水平和progesterone-treated乳腺。最后,我们评估是否发现分歧BALB / c和C57BL / 6小鼠菌株是由于上皮细胞的内在差异。方法:MPA的致癌效应C57BL / 6小鼠评估使用协议被证明是致癌的BALB / c小鼠。此外,BALB / c和C57BL / 6女性服用黄体酮或MPA 1或2个月,和乳腺切除组织学研究和免疫组织化学和免疫印迹评价ER和PR。激素水平测定等。孤立的乳腺上皮细胞被移植到清除脂肪垫21-day-old女性瑞士ν/ν老鼠或控制句话的动物。结果:MPA未能引起乳房癌或重要形态学C57BL / 6小鼠乳腺内的变化。ER-alpha和公关的表达同种型处女老鼠是令人惊讶的是更高的BALB / c比C57BL / 6乳腺,和两个受体表达下调progestin-treated BALB / c小鼠(P < 0.05)。公关同种型B水平低维珍控制老鼠和增加孕酮治疗后的菌株。 ER-beta expression followed a similar trend. No differences in hormone levels were found between strains. Surprisingly, the transplantation of the epithelial mammary gland cells of both strains into the cleared fat pads of Swiss (nu/nu) mice abolished the mammary gland morphologic differences and the ER and PR differences between strains. CONCLUSION: C57BL/6 mammary glands are resistant to MPA-induced carcinogenesis and to hormone action. MPA and progesterone have different effects on mammary glands. Low ER-alpha and PR-A levels in untreated mammary glands may be associated with a low-risk breast cancer profile. Although we cannot at this time rule out the participation of other, untested factors, our findings implicate the stroma as playing a crucial role in the strain-specific differential hormone receptor expression and hormone responsiveness.