组胺可以防止人类单核细胞凋亡。
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Soga F,岸本Katoh N, S
组胺可以防止人类单核细胞凋亡。
中国Exp过敏。2007年3月,37 (3):323 - 30。
- PubMed ID
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17359382 (在PubMed]
- 文摘
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背景:慢性过敏性皮炎(AD)与激活循环和浸润的单核细胞,单核细胞被认为发挥关键作用在广告建立慢性病变。组胺是一种重要的中介炎症和过敏反应。虽然新的免疫调节功能的组胺最近变得明显,组胺在单核细胞的寿命的影响尚不清楚。目的:在目前的研究中,我们调查了组胺对人类单核细胞的寿命的影响患者正常健康的捐赠者和广告。方法:单核细胞凋亡的诱导是通过血清剥夺,CD95 / Fas结扎或地塞米松组胺的存在,用膜联蛋白v和propidium iodide-staining。bcl - 2蛋白和激活caspase-3通过流式细胞仪测定。我们还研究了可溶性的影响,histamine-induced因素产生的单核细胞凋亡。此外,我们研究了单核细胞的细胞凋亡是否依赖于营地通路。结果:组胺预防单核细胞的凋亡诱导血清剥夺,CD95 / Fas结扎或地塞米松剂量和时间的方式。组胺对单核细胞的细胞凋亡的抑制作用被H2R拮抗剂,由H2R受体激动剂和模仿。 Histamine also up-regulated the expression of Bcl-2 and Mcl-1, and inhibited the activation of caspase-3. The culture supernatants from histamine-treated monocytes inhibited monocytic apoptosis, which was partly reversed by the removal of IL-10. Monocytes cultured with anti-IL-10 mAb and histamine did not exhibit an inhibitory effect on apoptosis. The histamine-induced anti-apoptotic effect was attenuated when monocytes were cultured in the presence of a cAMP inhibitor. CONCLUSIONS: These results indicate that the H2R signals induced by histamine allow monocytes to prolong their life span and infiltrate to the site of inflammation. This process may contribute to the establishment of chronic allergic disorders, such as AD.