Tn5的结构分析。
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Auerswald EA,路德维希G,夏勒H
Tn5的结构分析。
冷泉Harb电脑定量医学杂志。1981;45 Pt 1:107-13。
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6271452 (在PubMed]
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核苷酸序列已被确定为1.5 kb Tn5反向重复和他们的连接与独特的中部地区和宿主DNA。主要的发现源于这一分析是:1。整合Tn5伴随着9 bp宿主DNA的复制。2。Tn5发生的损失之间的交叉短,相应的核苷酸序列Tn5和主机之间的交界处附近的DNA。3所示。红外序列包含长,开平移阅读框架可能代码转座酶蛋白质。4所示。两个红外序列碱基变化不同。这个改变占这两个功能之间的差异观察IRL和IRR:它缩短了转座酶基因在线下阅读框,它提高了效率附近Km-resistance基因的启动子。 5. The NH2 terminus of the structural gene for the Km-resistance gene maps at the very left border of the unique region, i.e., very close to the end of IRL. These results support the view that the inverted repeats of Tn5 stem from two identical copies of an originally independently moving DNA element. In the transposon, one of these, IRL, seems to have evolved toward a close physical and functional linkage with the antibiotic-resistance gene.