细胞色素c - 550的作用通过反向遗传学研究和突变特征集胞藻属PCC 6803 sp。
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沈小,井上Vermaas W, Y
细胞色素c - 550的作用通过反向遗传学研究和突变特征集胞藻属PCC 6803 sp。
J生物化学杂志。1995年3月24日,270 (12):6901 - 7。
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7896839 (在PubMed]
- 文摘
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基因编码集胞藻属的细胞色素c - 550 sp。6803年PCC克隆基于氨基成熟的多肽序列。使用最可能的翻译起始密码子的基因编码160个氨基酸残基。这包括一个可分裂的氨基端领袖25残留的序列。这领袖序列Arg-Asn-Arg序列前立即裂解位点;这是原核生物转运肽的特征。比较这个序列的领袖序列光系统II-associated外在33-kDa蛋白质从同一藻青菌显示身份的13个25残留。这些结果表明,后脱辅基蛋白的合成,细胞色素c - 550运输到类囊体腔。使用克隆基因,插入和删除突变体集胞藻属sp。PCC 6803了。在缺乏细胞色素c - 550,两个突变体能够能光合自养的增长,但速度明显降低。阿特拉津bindng和免疫印迹分析表明,这些突变体per-chlorophyll基础上包含53 - 67%的光系统II与野生型相比。 The photosystem II-specific oxygen-evolving activity at saturating light intensity was reduced to about 40% of that in the wild type strain. Taken together, these results indicate that the cytochrome c-550 is transported into the thylakoid lumen and contributes to optimal functional stability of photosystem II in cyanobacteria. This supports our biochemical evidence that cytochrome c-550 is associated with the lumenal side of photosystem II as one of the extrinsic proteins enhancing oxygen evolution (Shen, J.-R., Ikeuchi, M., and Inoue, Y. (1992) FEBS Lett. 301, 145-149; Shen, J.-R., and Inoue, Y. (1993) Biochemistry 32, 1825-1832). Based on these results, the gene for cytochrome c-550 was named psbV. The possible evolutionary relationship among extrinsic proteins of the photosystem II donor side is discussed.