结构组织virulence-associated鼠疫杆菌的质粒。
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胡锦涛P,艾略特J, McCready P, Skowronski E,接着说下去! J,小林,布鲁巴克RR,加西亚E
结构组织virulence-associated鼠疫杆菌的质粒。
J Bacteriol。1998年10月,180 (19):5192 - 202。
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9748454 (在PubMed]
- 文摘
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完整的核苷酸序列和基因组织三毒力质粒的鼠疫杆菌KIM5测定。质粒pPCP1(9610个基点)的GC含量45.3%和编码两个先前已知的毒力因素,一个相关的蛋白质,和一个IS100副本。质粒pCD1(70504个基点)GC含量为44.8%。众所周知编码的重要致病因素,监管职能,multiprotein分泌系统由低钙响应刺激与其他两个共享鼠疫的物种对人类致病性(y .伪和y enterocolitica)。一个新的假基因,这是一个完整的基因y enterocolitica和y pseudotuberculosis-derived类似物,在pCD1被发现。它对应于脂蛋白YlpA编码。几个完整的和部分插入序列和/或转座子在pCD1还发现,以及六个假定的结构基因具有高同源性蛋白在其他yersiniae未知函数。基因的序列的复制pCD1高度同源的同源质粒在y伪和y enterocolitica,但是他们的定位在质粒明显不同于后者。质粒pMT1(100984个基点)GC含量为50.2%。它拥有两份IS100,相隔25 kb和相反的方向。 Adjacent to one of these IS100 inserts is a partial copy of IS285. A single copy of an IS200-like element (recently named IS1541) was also located in pMT1. In addition to 5 previously described genes, such as murine toxin, capsule antigen, capsule anchoring protein, etc., 30 homologues to genes of several bacterial species were found in this plasmid, and another 44 open reading frames without homology to any known or hypothetical protein in the databases were predicted.