监管网络的耐酸性基因在大肠杆菌。
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监管网络的耐酸性基因在大肠杆菌。
摩尔Microbiol。2003年5月,48 (3):699 - 712。
- PubMed ID
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12694615 (在PubMed]
- 文摘
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过度的反应调节器EvgA授予指数增长的大肠杆菌耐酸表型。这ydeP耐酸性部分被删除,yhiE或ydeO EvgA引起的基因超表达。微阵列分析确定两类操纵子(基因)。第一个类包含七个操纵子EvgA引起过度缺乏ydeO, AraC / XylS调节基因。第二个类包含12 YdeO过度引起的操纵子。操纵子在第二类EvgA引起的超表达只在ydeO的存在。EvgA可能直接移植操纵子在第一节课,和间接通过YdeO上调第二阶级操纵子。分析使用motif-finding程序alignace确定18 bp倒在上游六个区域重复主题直接受EvgA的七个操纵子。凝胶迁移转变化验显示特定的绑定EvgA的六个序列。引入突变的反向重复上游ydeP和b1500-ydeO导致减少EvgA-induced ydeP ydeO表达式和耐酸性。 These results suggest that EvgA binds to the inverted repeats and upregulates the downstream genes. Overexpression of YdeP, YdeO and YhiE conferred acid resistance to exponentially growing cells, whereas GadX overexpression did not. Microarray analysis also identified several GadX-activated genes. Several genes induced by overexpression of YdeO and GadX overlapped; however, yhiE was induced only by YdeO. The acid resistance induced by YdeO overexpression was abolished by deletion of yhiE, gadC, slp-yhiF, hdeA or hdeD, genes induced by YdeO overexpression, suggesting that several genes orchestrate YdeO-induced acid resistance. We propose a model of the regulatory network of the acid resistance genes.