正常肺发展RAIG1-deficient老鼠尽管独特肺epithelium-specific表达式。
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徐J,田J,夏皮罗SD
正常肺发展RAIG1-deficient老鼠尽管独特肺epithelium-specific表达式。
我和细胞杂志。2005年5月,32 (5):381 - 7。Epub 2005年1月27日。
- PubMed ID
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15677768 (在PubMed]
- 文摘
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RAIG1 2和3,GPCR5d代表一个新的亚孤儿G protein-coupled受体。RAIG1表达丰富,特别是在开发过程中肺和成年老鼠。在肺癌发展,RAIG1表达式E14.5开始,逐渐增加,在E18达到最高水平。维护高水平的表达在成人肺。鉴于其丰富lung-specific表达和维甲酸信号,我们假设RAIG1在肺上皮细胞分化发展中发挥作用。确定RAIG1函数和跟踪内生RAIG1空间表达,一个空的等位基因RAIG1生成和lacZ基因是“打”。Although expression was detected in both proximal and distal epithelium during embryogenesis, it became restricted to type I and type II pneumocytes and the most distal bronchiolar cells in postnatal lungs. This is the first gene known to have this unique epithelial cell expression pattern. Despite this high level of expression, targeted inactivation of Raig1 did not cause significant developmental defects. Epithelial cell differentiation was normal and lung structure was intact. Analysis of other family members demonstrated some overlapping embryonic expression of RAIG3 mRNA that could have led to functional redundancy in the single RAIG1 null mutant mouse.