进展性炎症犬牙龈组织纤溶酶原激活物和纤溶酶原激活物抑制剂2型表达增加。

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林德伯格P, Kinnby B, Lecander I, Lang NP, Matsson L

进展性炎症犬牙龈组织纤溶酶原激活物和纤溶酶原激活物抑制剂2型表达增加。

《口腔生物学》2001年1月;46(1):23-31。

PubMed ID

11163592 (PubMed视图

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摘要

尿激酶和组织型纤溶酶原激活剂(u—PA和t—PA)是将纤溶酶原转化为纤溶酶的丝氨酸蛋白酶，可降解基质蛋白并激活金属蛋白酶。PAs由特定的抑制剂(PAI—1和PAI—2)平衡。最近在人类牙龈组织中证实了t- PA和PAI- 2的局部产生。现在的目的是研究t- PA和PAI- 2在三种明确牙周条件下的犬牙龈组织中的产生和定位;临床健康的牙龈，慢性牙龈炎和结扎引起的附着丧失的初始阶段。在实验开始时，牙龈显示出明显的炎症迹象。强化口腔卫生21天后获得临床健康的牙龈。在部分牙齿颈部放置橡胶结扎器可导致附着丧失。从代表不同情况的区域取活检，并准备原位杂交和免疫组化。在临床健康的牙龈中，t- PA mRNA和抗原均在沟上皮和连接上皮的薄外层表达。 No t--PA signals or staining were seen in connective tissue. Both mRNA signaling and immunostaining for t--PA were stronger in chronic gingivitis. In areas with loss of attachment, t--PA mRNA as well as antigen were found in the sulcular and junctional epithelia to a similar degree as in gingivitis. Occasionally the connective tissue was involved, especially in connection with vessels. PAI--2 mRNA was seen in a thin outer layer of the sulcular and junctional epithelia in clinically healthy gingiva, but no signals were seen in connective tissue. PAI--2 antigen was found primarily in the outer layer of the sulcular and junctional epithelia. Some cells in the connective tissue were stained. In gingivitis, PAI--2 signals were mainly found in the same locations, but more intense and extending towards the connective tissue. Immunostaining was seen in the outer half of the sulcular and junctional epithelia as well as in the upper part of the connective tissue, close to the sulcular epithelium. In sites with loss of attachment, PAI--2 mRNA was found throughout the sulcular and junctional epithelia, as was the antigen, which stained intensely. No PAI--2 mRNA was seen in connective tissue; the antigen was found scattered, especially near vessels. This study shows that the expression of both t--PA and PAI--2 increases with experimental gingival inflammation in the dog, and furthermore, the two techniques demonstrate a strong correlation between the topographical distribution of the site of protein synthesis and the tissue location of the antigens for both t--PA and PAI--2. The distribution correlates well with previous findings in humans.

引用本文的药物库数据

药物靶点

药物	目标	种类	生物	药理作用	行动
Tenecteplase	纤溶酶原激活物抑制剂1	蛋白质	人类	未知的	不可用	细节