吸入丙酸氟替卡松对轻度哮喘支气管黏膜和血液淋巴细胞糖皮质激素受体mRNA的体内调节
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anderson O, Cassel TN, Gronneberg R, Bronnegard M, Stierna P, Nord M
吸入丙酸氟替卡松对轻度哮喘支气管黏膜和血液淋巴细胞糖皮质激素受体mRNA的体内调节
中华过敏临床免疫杂志，1999年4月;103(4):595-600。
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10200007 (PubMed视图］
- 摘要
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背景:糖皮质激素在体内调节糖皮质激素受体(GR)提供了一种调节靶细胞敏感性的手段。目的:我们试图确定丙酸氟替卡松(FP)对轻度哮喘受试者GR mRNA表达的体内调节。方法:10例特应性哮喘患者接受FP 250微克治疗，每天两次，持续4周。在治疗前后，患者接受纤维支气管镜检查，进行支气管内活检，并取样静脉血以测量GR mRNA水平。采用溶液杂交法对GR mRNA进行定量分析。此外，在FP治疗前后进行24小时尿皮质醇排泄和促肾上腺皮质激素测试。结果:GR mRNA的表达在个体间存在较高的差异。然而，我们检测到FP处理后支气管活检标本中GR mRNA水平显著降低(分别为36.6 +/- 23.1和25.0 +/- 10.9 amol GR mRNA/microg RNA;P < . 01)。在外周血淋巴细胞中，同源配体对GR的下调更为显著(分别为30.3 +/- 26.5和8.8 +/- 5 amol GR mRNA/microg RNA; P <.001), possibly reflecting differences in glucocorticoid sensitivity between tissues. A small but significant reduction of the 24-hour urinary cortisol excretion was observed (233 +/- 109 and 157 +/- 66 nmol/L, respectively; P <.01), whereas the feedback regulation of glucocorticoid synthesis by means of the hypothalamic-pituitary-adrenal axis as assessed by the adrenocorticotropic hormone test remained normal after treatment with FP. CONCLUSION: The results in this study confirm the potency of the inhaled corticosteroid FP and provide evidence for a considerable tissue-specific interindividual variation in the expression of the GR.