硫芴和萘在假单胞菌菌株代谢:完整的DNA序列上了萘降解途径。
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Denome SA,斯坦利,奥尔森,年轻的KD
硫芴和萘在假单胞菌菌株代谢:完整的DNA序列上了萘降解途径。
J Bacteriol。1993年11月,175 (21):6890 - 901。
- PubMed ID
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8226631 (在PubMed]
- 文摘
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从土壤分离,假单胞菌菌株C18,我们克隆和测序9.8 kb DNA片段编码dibenzothiophene-degrading酶。识别和指定doxABDEFGHIJ九个开放阅读框架。集体,我们将这些基因作为阿霉素通路。在核苷酸水平,doxABD相同ndoABC萘加双氧酶编码基因的假单胞菌putida。DoxG蛋白是97%相同NahC (1, 2-dihydroxynaphthalene加双氧酶)p . putida。DoxE身份与37% cis-toluene dihydrodiol脱氢酶。DoxF类似于醛脱氢酶的生物。预测DoxHIJ已知蛋白质蛋白质序列没有明显的相似之处。火焰离子化检测器气相色谱和质谱分析证实,阿霉素蛋白质转换萘水杨酸和转换菲1-hydroxy-2-naphthoic酸。doxI突变体转换萘trans-o-hydroxybenzylidenepyruvate,表明doxI蛋白类似NahE (trans-o-hydroxybenzylidenepyruvate hydratase-aldolase)。 Comparison of the DOX sequence with restriction maps of cloned naphthalene catabolic pathway (NAH) genes revealed many conserved restriction sites. The DOX gene arrangement is identical to that proposed for NAH, except that the NAH equivalent of doxH has not been recognized. DoxH may be involved in the conversion of 2-hydroxy-4-(2'-oxo-3,5-cyclohexadienyl)-buta-2,4-dienoat e to cis-o-hydroxybenzylidenepyruvate. doxJ encodes an enzyme similar to NahD (isomerase). Our findings indicate that a single genetic pathway controls the metabolism of dibenzothiophene, naphthalene, and phenanthrene in strain C18 and that the DOX sequence encodes a complete upper naphthalene catabolic pathway similar to NAH.