Src-dependent aprotein激酶C极微小/λ(aPKCiota /λ)需要酪氨酸磷酸化aPKCiota /λ与Rab2 pre-golgi中间体glyceraldehyde-3-phosphate脱氢酶。
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蒂斯达尔EJ、Artalejo CR
Src-dependent aprotein激酶C极微小/λ(aPKCiota /λ)需要酪氨酸磷酸化aPKCiota /λ与Rab2 pre-golgi中间体glyceraldehyde-3-phosphate脱氢酶。
生物化学杂志。2006年3月31日;281 (13):8436 - 42。Epub 2006 2月1。
- PubMed ID
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16452474 (在PubMed]
- 文摘
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需要小GTPase Rab2膜运输之间的内质网(ER)和高尔基氏复合体。Rab2 associates pre-Golgi中间体(也称为多孔管式集群;职业训练局)这类货物的顺行途径回收蛋白质检索ER。我们以前的研究已经表明Rab2刺激非典型蛋白激酶C极微小/λ(aPKCiota /λ)和glyceraldehyde-3-phosphate脱氢酶(GAPDH)招聘职业训练局。aPKCiota /λ和GAPDH直接绑定到Rab2和aPKCiota /λ和GAPDH交互。根据报告展示aPKCiota-Src交互和Src逆行通道的活动(Golgi-ER),研究启动学习是否Rab2也促进了Src招聘职业训练局。使用定量测定膜绑定,我们发现Rab2-stimulated Src膜协会剂量依赖性的方式。招募了Src结合aPKCiota /λ和GAPDH膜;然而,Src并不与Rab2交互。膜相关Src酪氨酸磷酸化aPKCiota /职业训练局λ。 To determine the consequence of aPKCiota/lambda tyrosine phosphorylation, the membrane binding assay was supplemented with the Src-specific tyrosine kinase inhibitor 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo(3,4-d)pyrimidine (PP2). Although Rab2, Src, and GAPDH recruitment was not affected, the Rab2-PP2-treated membranes contained a negligible amount of aPKCiota/lambda. Since Rab2 requires aPKCiota/lambda for the downstream recruitment of beta-coat protein (beta-COP) to VTCs, the Rab2-PP2-treated membranes were evaluated for the presence of beta-COP. Like aPKCiota/lambda, the membranes contained a negligible amount of beta-COP that was reflected by the drastic reduction in Rab2-dependent vesicle formation. These data suggest that Src-mediated tyrosine phosphorylation of aPKCiota/lambda facilitates aPKCiota/lambda association with Rab2-Src-GAPDH on VTCs, which is ultimately necessary for the downstream recruitment of beta-COP and release of Rab2-mediated retrograde-directed vesicles.