敏感自由洋地黄毒苷浓度测定方法使用洋地黄毒苷免疫测定:展示自由洋地黄毒苷浓度升高引起的digitoxin-phenytoin交互通过应用这些新技术。
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达斯古普塔,织女星AE,井,达塔P
敏感自由洋地黄毒苷浓度测定方法使用洋地黄毒苷免疫测定:展示自由洋地黄毒苷浓度升高引起的digitoxin-phenytoin交互通过应用这些新技术。
其他药物Monit。1999年12月,21 (6):625 - 30。
- PubMed ID
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10604823 (在PubMed]
- 文摘
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洋地黄毒苷非常强烈束缚血清白蛋白。虽然自由洋地黄毒苷药物活性,但它不是监视,因为缺乏足够的敏感技术。免费洋地黄毒苷的浓度无蛋白超滤液通常是低于洋地黄毒苷的检测极限分析。的改性技术是描述自由洋地黄毒苷可以使用商用例行监测分析。荧光偏振免疫分析法来确定总洋地黄毒苷浓度要求100 microL血清治疗300 microL甲醇沉淀蛋白。这是证明自由洋地黄毒苷可以很容易地通过增加测量100 microL甲醇300 microL超滤液,从而提高分析三个方面的敏感性。自由洋地黄毒苷浓度可以很容易地计算观测值除以3。试图只使用超滤液(没有甲醇添加)引起的显著偏差的结果,可能由于一个矩阵的问题。洋地黄毒苷的化学发光分析方法不需要任何样品预处理和只需要10 microL的血清。程序被修改和使用50 microL超滤液改善自由洋地黄毒苷的敏感性分析。 If the chemiluminescent assay is used to measure free digitoxin, the true free digitoxin concentration can be calculated by dividing the observed value by 4.3. The free digitoxin concentrations were comparable in eight patients receiving digitoxin as measured by both methods. To show an application of this technique, two serum pools were prepared from patients receiving digitoxin and supplemented with various concentrations of phenytoin. A significant increase in free digitoxin concentration was observed because of the displacement of digitoxin from protein binding sites by phenytoin.