尿毒症血清包含块digitoxin-valproic酸相互作用的抑制剂。
文章的细节
-
引用
复制到剪贴板 -
达斯古普塔,保罗,井
尿毒症血清包含块digitoxin-valproic酸相互作用的抑制剂。
医疗科学。2001年10月,322 (4):204 - 8。
- PubMed ID
-
11678517 (在PubMed]
- 文摘
-
背景:洋地黄毒苷和丙戊酸与血清白蛋白结合。因此,当目前同时在血清、洋地黄毒苷和丙戊酸争夺结合位点。我们研究digitoxin-valproic酸相互作用在正常和尿毒症血清。方法:荧光偏振分析被用于测量总洋地黄毒苷和总丙戊酸浓度。我们使用一个修改协议的测量自由洋地黄毒苷浓度的敏感性增强。我们补充2正常和2尿毒症池洋地黄毒苷然后整除的池与不同浓度的丙戊酸进一步补充。在37摄氏度孵化后2小时在水浴,标本被允许re-equilibrate在室温20分钟。免费的洋地黄毒苷浓度测定。我们也调查了digoxin-valproic酸交互使用正常,1尿毒症血清池。结果:我们观察到显著的自由洋地黄毒苷浓度在正常血清中丙戊酸的存在。 In contrast, we observed a slight decline in free digitoxin concentration in the presence of valproic acid in uremic sera. We speculated that uremic sera contained inhibitors that block digitoxin-valproic acid interaction and identified indoxyl sulfate as an inhibitor. However, another uremic compound, hippuric acid showed no inhibitory effect. Interestingly, we observed no significant interaction between digoxin and valproic acid in either normal or uremic serum pool. This is probably because of poor protein binding of digoxin. CONCLUSION: We conclude that valproic acid significantly displaces digitoxin from protein binding sites in normal serum. However, uremic sera contain inhibitors that block digitoxin-valproic acid interaction.