一级结构的反应中心Rhodopseudomonas sphaeroides。
文章的细节
-
引用
复制到剪贴板 -
威廉姆斯JC,施泰纳拉,视野中时G
一级结构的反应中心Rhodopseudomonas sphaeroides。
蛋白质。1986年12月,1(4):312 - 25所示。
- PubMed ID
-
3329732 (在PubMed]
- 文摘
-
介导的反应中心是一个pigment-protein复杂的初始光化步骤光合作用。伴序列的L、M和H子单元和核苷酸和推导的氨基酸序列的L和M结构基因Rhodopseudomonas sphaeroides此前就已确定。这里我们报告H亚基的顺序,完成的主要结构的决心从r . sphaeroides反应中心。基因的核苷酸序列编码H亚基是由双脱氧方法subcloning片段后单链M13噬菌体向量。这些信息被用来推导相应的多肽的氨基酸序列。的目的地的主要结构建立了H亚基的氨基和羧基末端序列的多肽。数据显示H亚基组成的260残留物,对应于一个分子量为28003。分子量为100858的反应中心计算子单元的主要结构和代数余子式。基因编码的反应中心的检查表明,密码子使用强烈偏向基码以G和c水疗法H亚基序列的分析,将揭示一段附近的疏水残基氨基酸;L和M亚基包含5个这样的延伸。 From a comparison of the sequences of homologous proteins found in bacterial reaction centers and photosystem II of plants, an evolutionary tree was constructed. The analysis of evolutionary relationships showed that the L and M subunits of reaction centers and the D1 and D2 proteins of photosystem II are descended from a common ancestor, and that the rate of change in these proteins was much higher in the first billion years after the divergence of the reaction center and photosystem II than in the subsequent billion years represented by the divergence of the species containing these proteins.