交互的脊髓灰质炎病毒受体CD155动力蛋白轻链Tctex-1脊髓灰质炎病毒发病机理及其含义。
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穆勒年代,曹X,威尔克R, Wimmer E
交互的脊髓灰质炎病毒受体CD155动力蛋白轻链Tctex-1脊髓灰质炎病毒发病机理及其含义。
生物化学杂志。2002年3月8日,277 (10):7897 - 904。Epub 2001年12月21日。
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11751937 (在PubMed]
- 文摘
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脊髓灰质炎病毒的细胞受体CD155 (PVR)是一种新的膜相关的创始成员immunoglobulin-like蛋白质,包括鼠标肿瘤相关抗原E4 (Tage4)和三种蛋白质称为“粘连蛋白”。Using a yeast two-hybrid screen we have discovered that the cytoplasmic domain of CD155 associates strongly and specifically with Tctex-1, a light chain of the dynein motor complex, the latter representing the major driving force for retrograde transport of endocytic vesicles and membranous organelles. We confirmed the interaction biochemically and by co-immunoprecipitation, and we mapped the Tctex-1 binding site to a SKCSR motif within the juxtamembrane region of CD155. Tctex-1 immunoreactivity was detected in mouse sciatic nerve and spinal cord (two tissues of central importance for poliovirus pathogenesis) in punctate, possibly vesicular, patterns. We propose that the cytoplasmic domain may target CD155-containing endocytic vesicles to the microtubular network. Neurotropic viruses like poliovirus, herpesvirus, rabies virus, and pseudorabies virus all utilize neuronal retrograde transport to invade the central nervous system. Association with Tctex-1 and, hence, with the dynein motor complex may offer an explanation for how poliovirus hijacks the cellular transport machinery to retrogradely ascend along the axon to the neuronal cell body.