代的一个集成的转录的地图BRCA2地区q12-q13 13号染色体上。
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沙发FJ, Rommens JM, Neuhausen SL Belanger C,杜蒙M亚伯K,贝尔R,浆果,Bogden R, Cannon-Albright L法L Frye C,海蒂T, Janecki T,江P, Kehrer R,勒布朗摩根富林明,McArthur-Morrison J, Meney D,杨爱瑾Y,彭Y,参孙C,施罗德M,斯奈德SC, Simard J, et al。
代的一个集成的转录的地图BRCA2地区q12-q13 13号染色体上。
基因组学。1996年8月15日,36 (1):86 - 99。
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8812419 (在PubMed]
- 文摘
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一个集成的方法包括物理映射,识别转录序列,计算分析基因组序列被用来生成一个详细的转录的地图1。0-Mb地区包含乳腺癌易感性位点BRCA2 q12-q13 13号染色体上。这个区域包含在基因间隔有界D13S1444和D13S310。检索从外显子序列扩增或混合选择程序分为物理间隔和随后克隆分为转录单位的重叠。重叠建立了直接杂交,cDNA库筛选,PCR cDNA链接(跳岛游),和/或序列比对。为了理解执行广泛的基因组测序转录单位组织。总共大约500 kb的基因组序列完成。RNA的转录单位进一步以杂交从一系列的人体组织。证据7基因,两个假定的伪基因,九个额外假定的转录单位。的一个转录单位最近被确定为BRCA2基因但其他小说未知函数的有限观察序列在公共数据库一致性。 One large gene with a transcript size of 10.7 kb showed significant similarity to a gene predicted by the Caenorhabditis elegans genome and the Saccharomyces cerevisiae genome sequencing efforts, while another contained a motif sequence similar to the human 2',3' cyclic nucleotide 3' phosphodiesterase gene. Several retrieved transcribed sequences were not aligned into transcription units because no corresponding cDNAs were obtained when screening libraries or because of a lack of definitive evidence for splicing signals or putative coding sequence based on computational analysis. However, the presence of additional genes in the BRCA2 interval is suggested as groups of putative exons and hybrid selected clones that were transcribed in consistent orientations could be localized to common physical intervals.