人类脾galaptin:物理化学特性。
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沙玛,Chemelli R,艾伦HJ
人类脾galaptin:物理化学特性。
生物化学。1990年6月5日,29(22):5309 - 14所示。
- PubMed ID
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2383549 (在PubMed]
- 文摘
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哺乳动物的脾脏以前报道含有beta-galactoside-binding凝集素(艾伦·h·J。希文斯基米。Palmberg, R。& DiCioccio r(1987)拱门。物化学。Biophys。256年,523 - 533]。本调查的目的是确定关系的人类脾galaptin其他beta-galactoside-binding凝集素在其他人类和动物组织确定。Galaptin亚基分子量14.5 kDa是唯一的这种类型的凝集素在人类发现脾脏所评估的sds - page, rp和免疫印迹分析。三个多肽pI的4.60,4.80,和4.85是纯化galaptin的等电点聚焦探测到,与主要的乐队拥有4.85π。紫外光谱分析表明没有假肢组和给A1%(1厘米),280 = 5.5。圆二色性的分析显示40%的存在β结构,大量的随机线圈,和10%的α螺旋结构。氨基酸组成非常类似于人类胎盘galaptin。 Amino acid sequence analyses were carried out on V8 protease, CNBr, and iodosobenzoic acid digestion fragments. A total of 94 residues were identified. All sequences determined could be aligned with placental galaptin sequences. We conclude that human splenic galaptin is identical with human placental galaptin. A related polypeptide of molecular mass approximately 14.5 kDa was found to be present in several different mammalian spleens as assessed by Western blot analysis using a monospecific polyclonal anti-human splenic galaptin antiserum.