隔离和分析人类46-kDa甘露糖6-phosphate受体基因。
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Klier HJ,冯Figura K, Pohlmann R
隔离和分析人类46-kDa甘露糖6-phosphate受体基因。
4月10欧元。1991;197(1):洋基。
- PubMed ID
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1849818 (在PubMed]
- 文摘
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从基因组文库在EMBL的3中,两个重叠克隆人类46-kDa甘露糖6-phosphate受体(MPR46)是孤立的,横跨整个编码序列。人类MPR46基因分布在12 kb,分为7个外显子(110 - 1573个基点)。所有内含子/外显子边界同意的共识序列拼接连接。外显子1编码一个5 '非翻译序列。ATG起始密码子开始于外显子2中第二个核苷酸。26个氨基酸残基的信号序列是紧随其后的是extracytoplasmic(腔)领域,延伸到外显子5。的跨膜域受体跨越外显子5和6和胞质域编码外显子6和7。后者域也为延长3 '翻译序列码。转录起始站点是由底漆扩展定义的。序列上游的帽子网站有很强的推广活动,包括在管家基因启动子的结构元素特征发现。 No correlation between the genomic organization and known protein domains of the MPR46 was apparent. Moreover, the sequence of about 150 amino acids within the luminal domain of MPR46, which is homologous to the 15 repeats that constitute the luminal domain of the 300-kDa mannose 6-phosphate receptor (MPR300), does not correlate with intron/exon borders. MPR46 and MPR300 have therefore diverged from a common ancestral gene before introduction of the present intron sequences.