58 k, microtubule-binding高尔基体蛋白,是一种formiminotransferase cyclodeaminase。

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Bashour, GS盛开

58 k, microtubule-binding高尔基体蛋白,是一种formiminotransferase cyclodeaminase。

生物化学杂志。1998年7月31日;273 (31):19612 - 7。

PubMed ID

9677387 (在PubMed

]

文摘

58 k之前确认为一只老鼠肝脏蛋白质结合微管体外和与高尔基体的胞质面体内(开花,S。Brashear, t . a(1989)生物。264年化学,16083 - 16092)。我们现在报告58 k是一个formiminotransferase cyclodeaminase (FTCD),双功能酶,催化tetrahydrofolate修改的两个连续的步骤5,10-methenyl tetrahydrofolate。比较免疫印迹使用几个单克隆抗体对58 k和多克隆抗体对鸡肝蛋白质(p60)具有类似的特性(Hennig D。尺度,s . J。男人,。穆勒,L . L。,德最大经济产量,J。克瑞斯,t . e .(1998)生物。化学。273年,19602 - 19611)证明精确co-purification蛋白质被所有抗体通过多个分离步骤,包括凝胶过滤和离子交换色谱法和蔗糖梯度超速离心法。八肽来自58 k显示高序列身份氨基酸序列预测的全长cDNA p60和猪的肝脏FTCD。此外,纯化58 k与formiminotransferase cyclodeaminase活动有关。基于这些集体结果,58 k被认为是大鼠肝脏FTCD版本。 Microtubules assembled from brain tubulin, but not from liver tubulin, were able to bind rat liver FTCD. Binding to brain microtubules is suspected to occur via polyglutamates that are added post-translationally to tubulin in brain, which was shown to contain very low levels of FTCD, but not to tubulin in liver, which was determined to be the richest tissue source, by far, of FTCD. The physiological significance of the microtubule binding activity of FTCD is thus called into question, but an association of FTCD with the Golgi apparatus has now been established.

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药物靶点

药物	目标	类	生物	药理作用	行动
四氢叶酸	Formimidoyltransferase-cyclodeaminase	蛋白质	人类	未知的	代数余子式	细节