调查一个碳代谢的调节拟南芥。

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李R,摩尔M,国王J

调查一个碳代谢的调节拟南芥。

植物细胞杂志。2003年3月,44 (3):233 - 41。

PubMed ID
12668769 (在PubMed
]
文摘

丝氨酸(Ser)生物合成C(3)植物可以通过几个途径发生。一个主要路线涉及tetrahydrofolate甘氨酸脱羧酶(四氢呋喃)端依赖活动复杂(环球数码创意,EC 2.1.1.10)和丝氨酸hydroxymethyltransferase (SHMT EC 2.1.2.1)甘氨酸(g)作为一个碳源(1 - c)。另一种THF-dependent通路包括C1-THF合酶与甲酸/ SHMT活动1 - c源。这里,我们一直在调查方面的监管这两个folate-mediated通路在拟南芥(l)Heynh。哥伦比亚使用两种方法。首先,转基因植物overexpressing甲酸脱氢酶(EC、1.2.1.2)被用来继续我们的先前的研究甲酸代谢外籍家庭佣工的功能。甲酸池大小大约是73 nmol (g弗兰克-威廉姆斯)(1)野生型拟南芥植物(WT);三个独立的转基因线已出池甲酸。转基因植物生产提供更多的(13)有限公司(2)((13)C)甲酸比WT植物但没有显著不同于WT植物Ser的合成。 We concluded that FDH has no direct role in the regulation of the above two pathways of Ser synthesis; the breakdown of formate to CO(2) by the FDH reaction is the primary and preferred fate of the organic acid in Arabidopsis. The ratio between the GDC/SHMT and C1-THF synthase/SHMT pathways of Ser synthesis from [alpha-(13)C]Gly and [(13)C]formate, respectively, in Arabidopsis shoots was 21 : 1; in roots, 9 : 1. In shoots, therefore, the pathway from formate plays only a small role in Ser synthesis; in the case of roots, results indicated that the 9 : 1 ratio was as a result of greater fluxes of (13)C through both pathways together with a relatively higher contribution from the C1-THF synthase/SHMT route than in shoots. We also examined the synthesis of Ser in a GDC-deficient mutant of Arabidopsis (glyD) where the GDC/SHMT pathway was impaired. Compared with WT, glyD plants accumulated 5-fold more Gly than WT after supplying [alpha-(13)C]Gly for 24 h; the accumulation of Ser from [alpha-(13)C]Gly was reduced by 25% in the same time period. On the other hand, the accumulation of Ser through the C1-THF synthase/SHMT pathway in glyD plants was 2.5-fold greater than that in WT plants. Our experiments confirmed that the GDC/SHMT and C1-THF synthase/SHMT pathways normally operate independently in Arabidopsis plants but that when the primary GDC/SHMT pathway is impaired the alternative C1-THF synthase/SHMT pathway can partially compensate for deficiencies in the synthesis of Ser.

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药物靶点
药物 目标 生物 药理作用 行动
四氢叶酸 丝氨酸hydroxymethyltransferase,胞质 蛋白质 人类
未知的
代数余子式
细节
四氢叶酸 丝氨酸hydroxymethyltransferase,线粒体 蛋白质 人类
未知的
代数余子式
细节