克隆、功能表达,和人类和小鼠的染色体定位gp180-carboxypeptidase想酶。
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石川T,村上K, Kido Y,吴建,年代Yazaki Y,原田F, Kuroki K
克隆、功能表达,和人类和小鼠的染色体定位gp180-carboxypeptidase想酶。
基因。1998年7月30日,215 (2):361 - 70。
- PubMed ID
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9714835 (在PubMed]
- 文摘
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我们之前报道,宿主细胞糖蛋白,gp180,结合鸭乙型肝炎病毒粒子,和编码羧肽酶基因家族的成员(Kuroki K。Eng F。石川,T。Turck C。,原田,F。Ganem D。,1995年。gp180,宿主细胞糖蛋白结合鸭乙型肝炎病毒粒子,编码羧肽酶基因家族的一员。生物。270年化学,15022 - 15028)。报告后,羧肽酶D (CPD)随后纯化牛垂体和特征作为小说羧肽酶E (CPE)例如酶,有许多共同的特征与鸭gp180(歌曲,L。弗里克,清醒,1995年。羧肽酶的纯化和表征,一种新型羧肽酶e酶,从牛垂体。生物。270年化学,25007 - 25013)。 CPD is now supposed to play an important role in a secretory pathway. To clarify the function of gp180 further, we have isolated and analyzed human and mouse homologues of duck gp180. cDNA clones derived from human HepG2 cells and mouse livers have been isolated on the basis of homology to the duck gp180. The suggested open reading frames of the human and mouse cDNA encode 1380 and 1377 amino acid proteins, respectively and have three carboxypeptidase homologous domains (A, B, and C). Domains A and B have completely conserved the residues known to have the enzymatic activity of carboxypeptidase, but domain C in each cDNA does not. Northern blotting revealed a ubiquitous tissue distribution of human gp180 mRNA with several transcript species. Expression of human gp180 cDNA in transfected 293T
细胞表现出在放射测定羧肽酶活性。人类和老鼠的同系物鸭与牛CPD gp180有许多共同的特征。荧光原位杂交显示,该基因编码人类gp180位于地区17 q11.2。