钾通道电流在大鼠间充质干细胞在细胞增殖及其可能的作用。

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王王SP,是的,罗RH,崔王寅,王H

钾通道电流在大鼠间充质干细胞在细胞增殖及其可能的作用。

中国Exp杂志杂志》2008年9月,35 (9):1077 - 84。doi: 10.1111 / j.1440-1681.2008.04964.x。2008年5月25日Epub。

PubMed ID

18505444 (在PubMed

]

文摘

间充质干细胞(MSC)显示相当大的再生和修复受损组织的承诺。然而,K +通道的电生理特性在MSC并不完善,对K +通道的作用在MSC增殖的调控。我们检测到三个不同的MSC向外电流:(i)延迟整流电流(翼(DR));(2)钙离子激活的K +电流(翼(Ca));和(iii)瞬态向外K +电流(Ito)。所有三个在场单独或结合在几乎所有细胞(90%)的调查。然而,10%的细胞不表达功能目前在生理潜力。逆转录-聚合酶链反应被用来识别信使rna与功能性离子电流有关。Kv1.2和Kv2.1与本土知识(博士);Slo和KCNN4与本土知识(Ca); and Kv1.4 and Kv4.3 were associated with Ito. The Kv channel blockers amiodarone, tetraethylammonium and verapamil, as well as increased extracellular K+ levels, inhibited proliferation of cultured MSC. In MSC treated with Kv channel blockers or an increased extracellular concentration of K+, the proportion of cells in the S phase decreased significantly and the proportion of cells in the G(0)/G(1) phase tended to increase, indicating that the cells were prevented from entering the S phase of the cell cycle. Our findings suggest that rat MSC heterogeneously express distinct types of K+ current, of which the voltage-gated IK(DR)-like K+ current is most common. Kv channel activity modulates the progression of the cell cycle, affecting the proliferation of MSC.

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药物靶点

药物	目标	类	生物	药理作用	行动
胺碘酮	HERG人类心脏K +通道(蛋白质组)	蛋白质组	人类	是的	抑制剂	细节