牛磺酸在哺乳动物的小脑:演示通过放射自显影法[3 h]牛磺酸和taurine-synthesizing酶抗体免疫细胞化学,cysteine-sulfinic酸脱羧酶。

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林Chan-Palay V, CT,那么坚强,山本M,吴司法院

牛磺酸在哺乳动物的小脑:演示通过放射自显影法[3 h]牛磺酸和taurine-synthesizing酶抗体免疫细胞化学,cysteine-sulfinic酸脱羧酶。

《美国国家科学院刊S a . 1982年4月,79 (8):2695 - 9。

PubMed ID

6953423 (在PubMed

]

文摘

牛磺酸神经元及其树突和轴突可视化在哺乳动物的小脑通过放射自显影法,体内注射后[(3)H]牛磺酸直接进入小脑皮层或深小脑核,在光和电子显微镜和免疫细胞化学与抗体水平cysteine-sulfinic酸脱羧酶(CSADCase;L-cysteine-sulfinate carboxylyase EC 4.1.1.29)。吸收和封存的[(3)H]牛磺酸贴上无数浦肯野细胞somata主树突和轴突;许多somata颗粒细胞、树突和平行纤维;星状、篮子和高尔基细胞;更大的神经元在所有深小脑核;最大的前庭外侧核神经元;,更很少,浦肯野细胞轴突神经纤维网的终端。标签在所有网站减少了注射前的小脑hypotaurine, p-chloromercuriphenylsulfonic酸,或beta-alanine,被马钱子碱几乎消除。采用免疫标记与多克隆抗体针对CSADCase酶负责hypotaurine从半胱氨酸亚磺酸和牛磺酸的合成半胱氨酸,有着相似的分布。 In electron micrographs, immunoreactivity within Purkinje cell somata and dendrites was localized to the Golgi apparatus, the inner plasma membrane, and condensed nonmembranous foci (120 nm in diameter) marked by clumps of peroxidase reaction product. Large Nissl bodies were usually not CSADCase immunoreactive. Numerous immunoreactive granule cells, dendrites, and parallel fibers were recognized. Pretreatment of the animals with colchicine increased the intensity of CSADCase immunoreactivity but did not change the number or distribution of labeled cells. These experiments indicate that taurine is synthesized and involved in a specific uptake process by cerebellar neurons. Neuroglial cells do not synthesize taurine but some neuroglia take up [(3)H]taurine. These findings call for a reexamination of the physiological function of taurine in the cerebellum. A hypothesis is proposed that taurine may be involved in the regulation of calcium, in dendritic spike generation, and in the inhibition of impulse propagation in major Purkinje cell dendrites.

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药物靶点

药物	目标	类	生物	药理作用	行动
半胱氨酸	半胱氨酸亚磺酸脱羧酶	蛋白质	人类	未知的	不可用	细节