Paraoxonase-2是一种广泛表达蛋白具有抗氧化特性并能防止细胞氧化修饰低密度脂蛋白。
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Ng CJ,哈林区的DJ, Gangopadhyay,哈马市,Grijalva VR,布米,Fogelman, Reddy圣
Paraoxonase-2是一种广泛表达蛋白具有抗氧化特性并能防止细胞氧化修饰低密度脂蛋白。
生物化学杂志。2001年11月30日;276 (48):44444 - 9。Epub 2001年9月28日。
- PubMed ID
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11579088 (在PubMed]
- 文摘
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载脂蛋白的氧化B-containing脂蛋白和细胞膜脂质被认为扮演不可或缺的角色发展的脂肪条纹病变,动脉粥样化形成的初始步骤。我们先前已经表明,两个antioxidant-like酶,paraoxonase(其)1和PON3高密度lipoprotein-associated蛋白质能够防止氧化修饰低密度脂蛋白(LDL) (Reddy, s T。哈林区,d J。Grijalva, V。Ng, C。哈马,S。Gangopadhyay,。施,d . M。露西,a·J。布,M。Fogelman, a . m . (2001) Arterioscler。Thromb。Vasc。医学杂志。542 - 547)。在目前的研究中,我们表明,PON2 (i)与高密度脂蛋白无关; (ii) has antioxidant properties; and (iii) prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL (MM-LDL), and inhibits the ability of MM-LDL to induce monocyte chemotaxis. The PON2 protein was overexpressed in HeLa cells using the tetracycline-inducible ("Tet-On") system, and its antioxidant capacity was measured in a fluorometric assay. Cells that overexpressed PON2 showed significantly less intracellular oxidative stress following treatment with hydrogen peroxide or oxidized phospholipid. Moreover, cells that overexpressed PON2 were also less effective in oxidizing and modifying LDL and, in fact, were able to reverse the effects of preformed MM-LDL. Our results suggest that PON2 possesses antioxidant properties similar to those of PON1 and PON3. However, in contrast to PON1 and PON3, PON2 may exert its antioxidant functions at the cellular level, joining the host of intracellular antioxidant enzymes that protect cells from oxidative stress.