抗菌活性的微生物鉴定评估肠外tildipirosin对食源性病原体和同桌的结肠的牛肉牛和猪。

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玫瑰,Pridmore,肖,威廉C,门格M, "年代,Ropke R, Nurnberger进行M

抗菌活性的微生物鉴定评估肠外tildipirosin对食源性病原体和同桌的结肠的牛肉牛和猪。

J兽医杂志。2016年6月,39 (3):277 - 86。doi: 10.1111 / jvp.12277。Epub 2015 11月5。

PubMed ID

26538405 (在PubMed

]

文摘

Tildipirosin(小费)是一种新型16-membered-ring大环内酯物授权治疗牛和猪的呼吸道疾病。pH值依赖大环内酯物的抗菌活性是众所周知的。考虑到越来越多的pH值在结肠内容牛肉牛和猪通常是pH值低于7.0,最低抑制浓度(MIC)的提示对食源性致病菌如弯曲杆菌杆菌(c), c .空肠和沙门氏菌血清和同桌的物种包括(大肠)粪肠球菌,大肠都有效,大肠杆菌测定标准(pH值7.3 + / - 1)或中性和微酸性条件。降低pH值从7.3到6.7导致增加中等收入国家的小费。除了麦克> 256 /毫升观察面部抗c .杆菌和肠球菌物种的族群,麦克风范围从2 - 8杯/毫升增加到64 - > 256杯/毫升为沙门氏菌和大肠杆菌,分裂到8 - 16个杯子/毫升从32 - 128杯/毫升为两个弯曲杆菌物种,从4-32杯子/毫升128 - > 256 /毫升为肠球菌物种。估计的抗菌活性,提示在牲畜的结肠内容推荐使用非肠道管理技巧(Zuprevo ((R))),并比较这麦克风在微酸性结肠pH值增加,我们开发的微生物鉴定和验证提示和使用这个测试所收集的粪便样本牛和猪。微生物的活性细胞腔的提示决心从稀释粪便水上层清液,使用标准曲线产生TIP-spiked粪便上层清液。量化的极限(定量限)提示1杯/毫升(ppm)。在牛的一项研究(n = 14), 3 28收集粪便样本24和48 h后处理被发现含有提示以上定量限浓度(1.3 - -1.8 ppm)。在另一个牛研究(n = 12) 8点收集粪便样本,24和48 h后处理,小费的定量限浓度高于4 8 h的样品(1.2 - -2.6 ppm)和一个24小时的样品(1.3 ppm)。 In a pig study (n = 12) with faecal samples collected 24, 48 and 72 h post-treatment, only one sample contained TIP above the LOQ (concentration 1.5 ppm). In another pig study (n = 12), with samples collected at 8, 24 48 and 96 h post-treatment, TIP concentrations were above the LOQ in one 8-h sample (1.1 ppm) and two 24-h samples (2.3 and 2.5 ppm). None of the 48-h and 96-h samples from these 4 studies contained measurable TIP concentrations. Thus, in cattle and pigs, only a small fraction of faecal samples collected up to 24 h postdosing contained measurable microbiologically active TIP, with its maximum limited to 2.6 mug/mL. This is several log2 dilution steps below the MICs of TIP against foodborne pathogens and commensals collected under acidic conditions comparable with those in the colonic contents and may explain a lack of intestinal dysbacteriosis with parenteral tildipirosin in livestock.

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药物

Tildipirosin