17 beta-trenbolone暴露程序幼虫青鳉的代谢障碍。

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Mizukami-Murata年代,Kishi-Kadota K, Nishida T

17 beta-trenbolone暴露程序幼虫青鳉的代谢障碍。

环境Toxicol。2015年6月4。doi: 10.1002 / tox.22158。

PubMed ID

26040664 (在PubMed

]

文摘

在这里,我们使用生理和转录组分析评估的影响17 beta-trenbolone (TB)在代谢上青鳉的早期阶段(Oryzias latipes)。在生理实验中,性逆转率不断增加结核病浓度比例(2 - 100 ng / L)和100%(男性)200 ng / L治疗组。结核病引起gonadosomatic指数显著增加的女性在60和100 ng / L的浓度。这些女性腹部肿胀和鸡蛋生产和生育率下降。显著增加观察这些雌性的身体质量指数。结核病导致男性生育能力下降,浓度> 20 ng / L,但没有观察到其他影响。在转录组(芯片)的实验中,幼虫受到结核7 d。分析使用KEGG Orthology数据库显示显著调节基因的主要类别包括“脂质代谢”和“多酮类化合物和萜类化合物代谢”。Thirteen genes (including those for hydroxymethylglutaryl-CoA synthase, cytoplasmic synthase, and lanosterol synthase) related to cholesterol biosynthesis via the mevalonate pathway were highlighted in these categories. Reverse transcriptase-polymerase chain reaction analyses were consistent with the microarray results, in terms of the direction and magnitude of change to gene expression. Among the downregulated genes, angiopoietin-like 4 and mitochondrial uncoupling protein 1, which are inversely correlated with obesity, were detected in the TB treatments. In conclusion, the results suggest that the exposure of females to TB during the early life stage may cause metabolic dysfunctions, including obesity and disrupted cholesterol synthesis. (c) 2015 Wiley Periodicals, Inc. Environ Toxicol, 2015.

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药物

Trenbolone