(二类人类组织相容性抗原的主要结构。2日沟通。氨基酸序列的氨基端179残留的alpha-chain HLA-Dw2 / DR2同种抗原(作者transl)]。
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杨C, Kratzin H, Gotz H, Thinnes FP,克鲁斯T, Egert G,保利E, Kolbel年代,Wernet P, Hilschmann N
(二类人类组织相容性抗原的主要结构。2日沟通。氨基酸序列的氨基端179残留的alpha-chain HLA-Dw2 / DR2同种抗原(作者transl)]。
霍普Seylers Z杂志化学。1982年6月,363 (6):671 - 6。
- PubMed ID
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6955253 (在PubMed]
- 文摘
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从lymphoblastoid纯合子细胞系(HLA-A3 3;B7 7;Dw2 2;DR2, 2) alpha-chain HLA-Dw2 / DR2抗原被一个完全孤立的化学过程。α-被与羟基磷灰石层析分离从beta-chain Na-dodecyl硫酸。在这里,我们描述的氨基酸序列alpha-chain 179位置。分子分为两个域不出现同源但表现出显著的同源性beta-chain (21.2%)。相似之处是在第二个(27%)比在第一(15.5%)领域,表明不同的进化关系两个部分。相比这两个域包含一个N-glycosidically beta-chain碳水化合物有关。蛋氨酸是定位只有在氨基,半胱氨酸只在c端域。 Only the latter can therefore be stabilized by a disulfide bridge. As with the beta-chain the regions around the cysteines show a remarkable similarity with the constant C-terminal domains of k-, lambda-, alpha-, gamma-, sigma-, epsilon- and mu-chains of immunoglobulins. Although to a considerably lesser extent, the alpha-chain preparation also shows a heterogeneity at the protein level. Since the employed cell-line is homozygous with regard to HLA-D/DR, our results indicate that at least two alpha-chain genes exist in the HLA-D/DR-region. Together with the already published sequence of the beta-chain, the extracellular part of an histocompatibility antigen of the HLA-D type is now known.