单电子还原丝裂霉素c的鼠肝:细胞色素p - 450的作用和NADPH-cytochrome p - 450还原酶。

文章的细节

引用

复制到剪贴板

虽然RM, van de海峡R,顶尖M, Vermeulen NP

单电子还原丝裂霉素c的鼠肝:细胞色素p - 450的作用和NADPH-cytochrome p - 450还原酶。

Xenobiotica。1990年9月,20 (9):967 - 78。

PubMed ID

2122607 (在PubMed

]

文摘

1。细胞色素p - 450的作用在单电子还原研究了丝裂霉素c在大鼠肝微粒体系统和纯化细胞色素p - 450的重组系统。形成过氧化氢的氧化还原循环减少丝裂霉素c在O2和烷基化p-nitrobenzylpyridine(平衡)在缺乏氧气被作为参数。2。与肝微粒体从3-methylcholanthrene (MC)和苯巴比妥(PB)预处理大鼠,反向I型差异光谱观察,表明之间的弱相互作用丝裂霉素c和细胞色素p - 450的底物结合位点。丝裂霉素c抑制氧化脱烷基化作用的氨基比林和ethoxyresorufin微粒体系统。3所示。在有氧条件下微粒体中的过氧化氢生产系统依赖于NADPH, O2和丝裂霉素c,并抑制细胞色素p - 450的抑制剂,甲吡酮和skf - 525 a。4所示。虽然纯化NADPH-cytochrome p - 450还原酶也有效地减少丝裂霉素c和O2的减少,微粒体系统和完全重建系统完整的细胞色素p - 450 b、p - 450 c和还原酶更有效。 5. Under anaerobic conditions in the microsomal systems both reduction of mitomycin c (measured as the rate of substrate disappearance) and the reductive alkylation of NBP were dependent on cytochrome P-450. 6. The relative rate of reduction of mitomycin c by purified NADPH-cytochrome P-450 reductase was lower than that by a complete microsomal system containing both cytochrome P-450 and a similar amount of NADPH-cytochrome P-450 reductase. 7. It is concluded that although NADPH-cytochrome P-450 reductase is active in the one-electron reduction of mitomycin c, the actual metabolic locus for the reduction of this compound in liver microsomes under a relatively low O2 tension is more likely the haem site of cytochrome P-450.

DrugBank数据引用了这篇文章

药物酶

药物	酶	类	生物	药理作用	行动
丝裂霉素	NADPH -细胞色素P450还原酶	蛋白质	人类	未知的	底物	细节