l -蛋氨酸对酿酒酵母C代谢的调控。

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Lor KL, Cossins EA

l -蛋氨酸对酿酒酵母C代谢的调控。

生物化学杂志，1972年12月;130(3):773-83。

PubMed ID

4198357 (PubMed视图

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摘要

1.采用干酪乳杆菌(A.T.C.C. 7469)和酿酒小球菌(A.T.C.C. 8081)的微生物法测定了啤酒酵母(A.T.C.C. 9763)好氧培养物中叶酸衍生物的浓度。在缺乏l-蛋氨酸的培养基中培养的细胞比在添加2.5mumol l-蛋氨酸/ml的相同培养基中培养的细胞含有更高浓度的叶酸衍生物。在生长培养基中添加l-蛋氨酸也降低了高共轭衍生物的浓度。2.从这些条件下生长的细胞中提取的deae -纤维素柱层析显示，甲基化四氢叶酸的浓度因补充蛋氨酸而急剧下降。甲酰化和未取代衍生物的浓度也有较小的下降。3.比较生长6h时有l-蛋氨酸(2.5mumol/ml)和无l-蛋氨酸(2.5mumol/ml)时C(1)代谢的4种酶的浓度。甲酰四氢叶酸合成酶、亚甲基四氢叶酸还原酶和丝氨酸羟甲基转移酶的活性未受影响，但5-甲基四氢叶酸-同型半胱氨酸甲基转移酶的活性约降低。 65% when l-methionine was supplied. The activities of 5-methyltetrahydrofolate-homocysteine methyltransferase, serine hydroxymethyltransferase and formyltetrahydrofolate synthetase were not appreciably altered by l-methionine in vitro. In contrast this amino acid was found to inhibit the activity of methylenetetrahydrofolate reductase. 4. Feeding experiments employing sodium [(14)C]formate indicated that cells grown in the presence of exogenous methionine, although having less ability to convert formate into methionine, readily incorporated (14)C into serine and the adenosyl moiety of S-adenosylmethionine. 5. It is suggested that exogenous l-methionine controls C(1) metabolism in Saccharomyces principally by regulation of methyl-group biogenesis within the folate pool.

引用本文的药物库数据

药物酶

药物	酶	种类	生物	药理作用	行动
甲硫氨酸	Methylenetetrahydrofolate还原酶	蛋白质	人类	未知的	抑制剂	细节