磷脂酰肌醇的亚细胞位置4-kinase亚型。
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黄K,迈耶斯ddR,坎特LC
磷脂酰肌醇的亚细胞位置4-kinase亚型。
生物化学杂志。1997年5月16日;272 (20):13236 - 41。
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9148941 (在PubMed]
- 文摘
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磷脂酰肌醇(PtdIns) 4-kinase PtdIns-4-P的催化合成的前体脂质第二信使生成数组通过额外的磷酸化PtdIns-4-P 5-kinase和PtdIns 3-kinase。PtdIns 4-kinase活动从高等真核生物酵母是守恒的。多种亚型的哺乳动物PtdIns 4-kinase净化,和活动已发现在几乎所有的亚细胞位置。我们之前报道的第一个哺乳动物的克隆和表征PtdIns 4-kinase名为PI4Kalpha (Wong K。坎特,l . c(1994)生物。269年化学,28878 - 28884)。或者拼接形式的PI4Kalpha也已确定的几个来源包括牛脑(Gehrmann, T。Vereb, G。施密特,M。Klix D。迈耶,h。E。Varsanyi, M。,Heilmeyer l . M。小(1996)Biochim。Biophys。 Acta 1311, 53-63). Recently we isolated a distinct human PtdIns 4-kinase gene, named PI4Kbeta, that encodes an enzyme that is wortmannin sensitive (Meyers, R., and Cantley, L. C. (1997) J. Biol. Chem. 272, 4384-4390). Here we report the locations of these enzymes and provide evidence for other yet unidentified isoforms present in specific organelles. PI4Kalpha is mostly membrane-bound and located at the endoplasmic reticulum; whereas PI4Kbeta is in the cytosol and also present in the Golgi region. Neither of these isoforms accounts for the major type II PtdIns 4-kinase activity detected in the lysosomes and plasma membrane fraction.