Cell-cycle-dependent改变高度磷酸化的核仁的蛋白质p130与nucleologenesis相关联。
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Pai CY,陈港元,张文雄霍奇金淋巴瘤,叶NH
Cell-cycle-dependent改变高度磷酸化的核仁的蛋白质p130与nucleologenesis相关联。
108年5月,J细胞科学。1995 (Pt 5): 1911 - 20。
- PubMed ID
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7657714 (在PubMed]
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我们还发现了一个新人类核仁的磷蛋白质p130 (130 kDa)使用的策略选择单克隆抗体核蛋白质在细胞周期振荡。p130相间点缀的方式核仁本地化。完成提取p130需要高浓度的盐(0.5 M氯化钠)表明它牢牢绑定到核仁的组件通过离子相互作用。p130严重磷酸化,因为碱性磷酸酶处理的净化p130转换为95 kDa产品;这是进一步支持细胞的体外示范磷酸酶和酪蛋白激酶ⅱ活动负责这两种形式的交换。有丝分裂细胞的提取较低浓度的p130相比的间期细胞表明在有丝分裂p130的比例可能会退化。此外,所有剩余的p130有丝分裂细胞进一步磷酸化,由cdc2激酶可能导致增加其溶解度,分散在整个细胞质。因此,在中期和后期p130细胞被免疫荧光显微镜无法被检测到。在末期,p130又聚合成粒状结构,类似于prenucleolar尸体。这些颗粒迁移从核浆在g1期年初核仁。 Actinomycin D was able to induce segregation of p130-containing granules into the nucleoplasm, similar to the well-known behavior of the fibrillarin-containing granules, indicating that p130 is localized in the dense fibrillar component, a subnucleolar region for pre-rRNA synthesis and processing. The cDNA sequence of p130 revealed a remarkable feature, that a serine-rich stretch interspersed with acidic residues is repeated ten times. Such a characteristic is shared with a rat nucleolar phosphoprotein Nopp140, which is thought to shuttle between the nucleolus and the cytoplasm. Although p130 shows 74% identity to Nopp140, our observations suggest that during mitosis the functions of p130 are related to nucleologenesis.