蛋白质和碳水化合物结构分析重组可溶性CD4受体的质谱分析。
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卡尔SA, Hemling我Folena-Wasserman G,甜RW, Anumula K,巴尔JR。哈迪MJ,泰勒P
蛋白质和碳水化合物结构分析重组可溶性CD4受体的质谱分析。
生物化学杂志。1989年12月15日;264 (35):21286 - 95。
- PubMed ID
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2592374 (在PubMed]
- 文摘
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可溶性的主要结构形式的CD4受体(sCD4)表示在中国仓鼠卵巢细胞已经被质谱肽图证实和串联质谱分析。这些研究证实了369 - 95%的氨基acid-long序列,建立了翻译的忠实的氨基和羧基终端包括缺乏“衣衫褴褛的结束。”The arrangement of the three disulfide bonds in recombinant sCD4 was also established by mass spectrometry and comparative high performance liquid chromatography mapping and shown to be identical to that expected from previous studies of intrachain disulfide bonding in T4 antigens derived from sheep and mouse. No other arrangements of disulfides were detected. Carbohydrate mapping by mass spectrometry was used to establish that both potential Asn-linked glycosylation sites in sCD4 (Asn271 and Asn300) have oligosaccharides attached. Structural characterization by mass spectrometry and methylation analysis of the heterogeneous family of oligosaccharides at each of the specific attachment sites indicates that the major components of both families of oligosaccharides have the following biantennary structures: (Formula, see text) where m + n = 0-2, and x = 0,1. Minor carbohydrate components having three N-acetylneuraminic acid (NeuAc) groups and an additional hexose-hexosamine unit were detected by high performance anion-exchange chromatography.