H+/肽共转运体家族新成员PEPT 2的分子克隆。
文章的细节
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引用
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刘伟,梁瑞,Ramamoorthy S,费玉杰,Ganapathy ME, Hediger MA, Ganapathy V, Leibach FH
H+/肽共转运体家族新成员PEPT 2的分子克隆。
生物化学学报1995 5月4日;1235(2):461-6。
- PubMed ID
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7756356 (PubMed视图]
- 摘要
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已知哺乳动物肾脏表达小肽和药理活性氨头孢菌素的特异性运输系统。该系统由跨膜电化学H+梯度供电。最近,一个H(+)偶联肽转运体已经从兔和人的肠道中克隆出来(Fei et al. (1994) Nature 368, 563-566;梁等,J.生物学。化学。,出版中)。功能研究已经确定,肾肽运输系统是类似的,但不完全相同的肠对应物。因此,为了分离肾脏H+/肽共转运体cDNA,我们利用来自兔肠道H+/肽共转运体cDNA的探针筛选了人肾脏cDNA文库。这导致分离出一个长2190 bp的开放阅读框的阳性克隆。预测的蛋白质由729个氨基酸组成。 Hydropathy analysis of the amino acid sequence indicates the presence of twelve putative transmembrane domains. The primary structure of this protein exhibits 50% identity and 70% similarity to the human intestinal H+/peptide cotransporter. Functional expression of the kidney cDNA in HeLa cells results in the induction of a H(+)-coupled transport system specific for small peptides and aminocephalosporins. Reverse transcription-coupled polymerase chain reaction demonstrates that the cloned transporter is expressed in human kidney but not in human intestine. This transporter, henceforth called PEPT 2, represents a new member in the growing family of H(+)-coupled transport systems in the mammalian plasma membrane.
引用本文的药物库数据
- 药物转运蛋白
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药物 转运体 种类 生物 药理作用 行动 头孢氨苄 溶质载体家族15个成员2 蛋白质 人类 未知的抑制剂细节 - 多肽
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名字 UniProt ID 溶质载体家族15个成员2 Q16348 细节