描述VIM-2, carbapenem-hydrolyzing金属-β-内酰胺酶及其基因的质粒,integron-borne铜绿假单胞菌临床孤立法国。
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Poirel L, Naas T,尼古拉斯·D, L夹头,Bellais年代,卡瓦略JD,高加索P
描述VIM-2, carbapenem-hydrolyzing金属-β-内酰胺酶及其基因的质粒,integron-borne铜绿假单胞菌临床孤立法国。
Antimicrob代理Chemother。2000年4月,44 (4):891 - 7。
- PubMed ID
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10722487 (在PubMed]
- 文摘
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铜绿假单胞菌COL-1被认定的血培养39-year-old-woman对待imipenem在马赛,法国,1996年。这株耐beta-lactams,包括ureidopenicillins ticarcillin-clavulanic酸、头孢吡肟、头孢他啶,imipenem, meropenem,但仍然容易受到monobactam aztreonam。carbapenem-hydrolyzing的铜绿假单胞菌COL-1 beta-lactamase基因的克隆、测序,并在大肠杆菌DH10B表示。推导出266 -氨基酸蛋白Ambler乙级beta-lactamase,与32%的氨基酸身份B-II从蜡样芽胞杆菌;革兰氏阴性杆菌31% IMP-1从几个在日本,包括铜绿假单胞菌;27%的CcrA脆弱拟杆菌;24%的泄漏Chryseobacterium脑膜炎败;24%的IND-1 Chryseobacterium indologenes;21%的CphA-1气单胞菌属hydrophila;和11%的l - 1 Stenotrophomonas maltophilia。 It was most closely related to VIM-1 beta-lactamase recently reported from Italian P. aeruginosa clinical isolates (90% amino acid identity). Purified VIM-2 beta-lactamase had a pI of 5.6, a relative molecular mass of 29.7 kDa, and a broad substrate hydrolysis range, including penicillins, cephalosporins, cephamycins, oxacephamycins, and carbapenems, but not monobactams. As a metallo-beta-lactamase, its activity was zinc dependent and inhibited by EDTA (50% inhibitory concentration, 50 microM). VIM-2 conferred a resistance pattern to beta-lactams in E. coli DH10B that paralleled its in vitro hydrolytic properties, except for susceptibility to ureidopenicillins, carbapenems, and cefepime. bla(VIM-2) was located on a ca. 45-kb plasmid that in addition conferred resistance to sulfamides and that was not self-transmissible either from P. aeruginosa to E. coli or from E. coli to E. coli. bla(VIM-2) was the only gene cassette located within the variable region of a novel class 1 integron, In56, that was weakly related to the bla(VIM-1)-containing integron. VIM-2 is the second carbapenem-hydrolyzing metalloenzyme characterized from a P. aeruginosa isolate outside Japan.