隔离、序列和表达人类角蛋白K5基因转录调节角质和洞察成对控制。
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Lersch R, Stellmach V,股票C, Giudice G, Fuchs E
隔离、序列和表达人类角蛋白K5基因转录调节角质和洞察成对控制。
摩尔细胞杂志。1989年9月,9 (9):3685 - 97。
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2476664 (在PubMed]
- 文摘
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mitotically活性基底层最复层鳞状上皮细胞表达角蛋白10至30%的总蛋白。两个在这些细胞特异表达角蛋白II型角蛋白K5 (58 kilodaltons)及其相应的合作伙伴,I型角蛋白K14 (50 kilodaltons),这两个8-nm丝的形成至关重要。解剖分子机制的协同调节两个角蛋白是一种重要的第一步,了解表皮分化和设计推广者,使交付和外源基因的表达产品的复层鳞状上皮细胞,如皮肤。以前,我们报道基因的序列编码人类K14 (d . Marchuk s McCrohon e·福克斯,细胞39:491 - 498,1984;Marchuk et al ., Proc。国家的。学会科学。美国82:1609 - 1613,1985)。我们现在人类K5孤立和特征基因编码。这个基因的序列编码的部分匹配完美与部分K5 cDNA序列从人工培养的表皮获得库(r . Lersch和e·福克斯,摩尔。细胞。生物8:486 - 493,1988),和基因转染的研究表明,基因功能。 Nuclear runoff experiments demonstrated that the K5 and K14 genes were both transcribed at dramatically higher levels in cultured human epidermal cells than in fibroblasts, indicating that at least part of the regulation of the expression of this keratin pair is at the transcriptional level. When the K5 gene was transfected transiently into NIH 3T3 fibroblasts, foreign expression of the gene caused the appearance of endogenous mouse K14 and the subsequent formation of a keratin filament array in the cells. In this case, transcriptional changes did not appear to be involved in the regulation, suggesting that there may be multiple control mechanisms underlying the pairwise expression of keratins.