bla传播(CTX-M-14)是主要由IncK质粒在大肠杆菌传播phylogroups, B1,在西班牙和D。
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瓦尔韦德,广州R, Garcillan-Barcia MP, Novais,加兰JC,阿尔瓦拉多,de la Cruz F, F Baquero, Coque TM
bla传播(CTX-M-14)是主要由IncK质粒在大肠杆菌传播phylogroups, B1,在西班牙和D。
Antimicrob代理Chemother。2009年12月,53(12):5204 - 12所示。doi: 10.1128 / AAC.01706-08。Epub 2009年9月28日。
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19786598 (在PubMed]
- 文摘
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自2000年首次描述,CTX-M-14已经成为最广泛extended-spectrum beta-lactamases在西班牙。在目前的大肠杆菌多级人口基因研究涉及phylogroups的特性,克隆,质粒,和遗传平台,61隔离来自16个住院病人和40个门诊病人和健康志愿者恢复从2000年到2005年进行了分析。克隆亲缘(XbaI pulsed-field凝胶电泳(但是脉冲场凝胶电泳的出现)类型,系统组,茎序列类型[MLST])建立了标准方法。分析转移质粒(I-CeuI;S1核酸酶;限制片段长度多态性分析;和RNA干扰分析、复制酶和relaxase)是由PCR测序和杂交。bla (CTX-M-14)的基因环境的基础上以PCR相关的已知结构(ISEcp1, IS903 ISCR1)。隔离主要是恢复病人在社区(73.8%;与尿路感染(62.2%;45/61) 28/45). They were clonally unrelated by PFGE and corresponded to phylogenetic groups A (36.1%), D (34.4%), and B1 (29.5%). MLST revealed a high degree of sequence type (ST) diversity among phylogroup D isolates and the overrepresentation of the ST10 complex among phylogroup A isolates and ST359/ST155 among phylogroup B1 isolates. Two variants of bla(CTX-M-14) previously designated bla(CTX-M-14a) (n = 59/61) and bla(CTX-M-14b) (n = 2/61) were detected. bla(CTX-M-14a) was associated with either ISEcp1 within IncK plasmids (n = 27), ISCR1 linked to an IncHI2 plasmid (n = 1), or ISCR1 linked to IncI-like plasmids (n = 3). The bla(CTX-M-14b) identified was associated with an ISCR1 element located in an IncHI2 plasmid (n = 1) or with ISEcp1 located in IncK (n = 1). The CTX-M-14-producing E. coli isolates in our geographic area are frequent causes of community-acquired urinary tract infections. The increase in the incidence of such isolates is mostly due to the dissemination of IncK plasmids among E. coli isolates of phylogroups A, B1, and D.