结构、功能和immunolocalization proton-coupled氨基酸的转运体(hPAT1) Caco-2人类肠道细胞线。

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陈Z,范YJ,安德森厘米,KA, Miyauchi年代,黄W, Thwaites DT, Ganapathy V

结构、功能和immunolocalization proton-coupled氨基酸的转运体(hPAT1) Caco-2人类肠道细胞线。

杂志。2003年1月15日,546 (Pt 2): 349 - 61。

PubMed ID

12527723 (在PubMed

]

文摘

的人类orthologue H(+)耦合的氨基酸转运蛋白(hPAT1)克隆人类肠细胞系Caco-2及其功能特征评估在哺乳动物细胞异种的表达系统。克隆hPAT1由476氨基酸和展览85%与鼠PAT1身份。在各种人体组织检查吸干,北部PAT1 mRNA表达最主要在肠道。当表示不同的哺乳动物细胞,hPAT1介导运输α- (methylamino)异丁酸(MeAIB)。cDNA-induced运输Na(+)是独立的,但被一个内在激励指导H(+)梯度。hPAT1与甘氨酸,丙氨酸,L-proline alpha-aminoisobutyrate (AIB)和gamma-aminobutyrate (GABA),正如从竞争直接传输测量和实验与MeAIB运输衬底。hPAT1也认识到D-isomers丙氨酸和脯氨酸。丝氨酸和半胱氨酸,尽管L-isomers不与hPAT1任何重要程度上,相应的D-isomers被公认为基质。脯氨酸和丙氨酸、L -和D-isomers的亲和力很相似。然而,半胱氨酸和丝氨酸,D-isomers显示6 - 8番更高的亲和力比相应的L-isomers hPAT1。 These functional characteristics of hPAT1 closely resemble those that have been described previously for the H(+)-coupled amino acid transport system in Caco-2 cells. Furthermore, there was a high degree of correlation (r(2) = 0.93) between the relative potencies of various amino acids to inhibit the H(+)-coupled MeAIB transport measured with native Caco-2 cells and with hPAT1 in the heterologous expression system. Immunolocalization studies showed that PAT1 was expressed exclusively in the apical membrane of Caco-2 cells. These data suggest that hPAT1 is responsible for the H(+)-coupled amino acid transport expressed in the apical membrane of Caco-2 cells.

DrugBank数据引用了这篇文章

药物转运蛋白

药物	转运体	类	生物	药理作用	行动
(α)- methylamino异丁酸	Proton-coupled氨基酸转运体1	蛋白质	人类	未知的	不可用	细节

多肽

的名字	UniProt ID
Proton-coupled氨基酸转运体1	Q7Z2H8	细节