动力学分析的雄烯二酮5 alpha-reductase人类前列腺上皮和基质。
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weis H, Krieg M
动力学分析的雄烯二酮5 alpha-reductase人类前列腺上皮和基质。
类固醇。1997 Aug-Sep; 62 (8): 589 - 94。doi: 10.1016 / s0039 - 128 x (97) 00042 - 1。
- PubMed ID
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9432753 (在PubMed]
- 文摘
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在人类前列腺癌、各种androgen-metabolizing酶存在。在这些酶中,睾酮5 alpha-reductase似乎占主导地位。然而,雄烯二酮也是一个潜在的前列腺5 alpha-reductase衬底。解决的问题在多大程度上减少androstanedione雄烯二酮的发生,本研究详细描述了动力学特征(公里和Vmax)和可能的年龄相关性变化的酶介入人类前列腺上皮和基质。在正常前列腺(NPR),意味着公里(nM)和Vmax (pmol / mg protein.h)是双重的高基质(公里,211;Vmax, 130)比上皮(公里,120;Vmax 56),而在良性前列腺增生(BPH),意味着公里(nM;意思是+ / - SEM)和Vmax (pmol / mg protein.h;的意思是+ / - SEM)高出6倍在基质(668 + / - 121公里;Vmax 415 + / - 73)比上皮(120 + / - 10公里; Vmax, 73 +/- 8). In BPH, those differences between epithelium and stroma were highly significant (p < 0.001). However, the efficiency ratios (Vmax/Km) of neither BPH nor NPR showed any significant differences between epithelium (NPR, 0.47; BPH, 0.62 +/- 0.06) and stroma (NPR, 0.70; BPH, 0.63 +/- 0.05). With respect to age-related changes, only stroma showed a significant increase of Km (p < 0.01) and Vmax (p < 0.05) with age. In summary, in both epithelium and stroma of the human prostate, a 5 alpha-reductase converts in measurable amounts androstenedione to androstanedione. The kinetic data were, in part, different between epithelium and stroma; the reason for this difference remains unclear. In comparison to other metabolic conversions, such as testosterone to dihydrotestosterone and androstenedione to testosterone, it is unlikely that, in the human prostate, the adrenal androgen androstenedione contributes significantly to the formation of testosterone and, further, of dihydrotestosterone.
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