Nectin4/PRR4,一个新的与afadin相关的nectin家族成员,通过V域相互作用与nectin1/PRR1进行反式相互作用。
文章的细节
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引用
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雷蒙N,法布尔S,勒科克E,阿德莱德J,杜布勒伊P,洛佩兹M
Nectin4/PRR4,一个新的与afadin相关的nectin家族成员,通过V域相互作用与nectin1/PRR1进行反式相互作用。
生物化学杂志,2001 11月16日;276(46):43205-15。doi: 10.1074 / jbc.M103810200。Epub 2001 9月5日。
- PubMed ID
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11544254 (PubMed视图]
- 摘要
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树胶是粘附分子,参与上皮细胞和内皮细胞连接的组织,并作为单纯疱疹病毒进入的受体。它们属于免疫球蛋白超家族,是脊髓灰质炎病毒受体(PVR/CD155)的同源物,也被命名为脊髓灰质炎病毒受体相关(PRR)蛋白。我们发现了一个名为nectin4的新成员。人类和小鼠nectin4的肽序列具有92%的同源性,至于其他成员,外膜结构域由V, C, C型的三个免疫球蛋白样结构域组成。与其他连接素分子相比,连接素4转录本的检测主要局限于人类组织中的胎盘。在小鼠中表达更广泛,有趣的是,在小鼠胚胎发生的第11、15和17天检测到nectin4。Nectin4通过其羧基端细胞质序列与f -肌动蛋白相关分子afadin相互作用。这两种分子在MDCKII上皮细胞系中以钙粘蛋白为基础的粘附连接处共定位。nectin是一种嗜同性粘附分子,最近研究发现nectin3/nectin1和nectin3/nectin2之间存在嗜异性相互作用。我们证实了这些反式相互作用,并将nectin3描述为PVR/CD155配体。 By means of several approaches, we report on the identification of nectin4 as a new ligand for nectin1. First, a soluble chimeric recombinant nectin4 ectodomain (nectin4-Fc) trans-interacts with cells expressing nectin1 but not with cells expressing nectin2, nectin3, or PVR/CD155. Conversely, nectin1-Fc binds to cells expressing nectin4. Second, nectin1-Fc precipitates nectin4 expressed in COS cells. Third, reciprocal in vitro physical interactions were detected between nectin4-Fc and nectin1-Fc. The nectin4-Fc/nectin4-Fc interaction was detected suggesting that nectin4 exhibits both homophilic and heterophilic properties. Using the same approaches we demonstrate, for the first time, that the V domain of nectin1 acts as a major functional region involved in trans-heterointeraction with nectin4 and also nectin3.