过氧物酶体脂肪段微分调节的基因编码proliferator-activated receptor-gamma coactivator-1alpha和1β在人类骨骼肌细胞分化的体外。
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小马,小马K,哈斯C, weis M, Machicao F,哈林
过氧物酶体脂肪段微分调节的基因编码proliferator-activated receptor-gamma coactivator-1alpha和1β在人类骨骼肌细胞分化的体外。
Diabetologia。2005年10月,48 (10):2115 - 8。doi: 10.1007 / s00125 - 005 - 1895 - z。Epub 2005年8月17日。
- PubMed ID
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16132959 (在PubMed]
- 文摘
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目的/假说:转录辅激活过氧物酶体proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha)增强新陈代谢相关的途径,如糖质新生,脂肪酸氧化,产热、氧化磷酸化和线粒体生物起源。自调节基因编码的表达PGC-1alpha (PPARGC1A)营养/代谢物尚未详细评估,本研究的目的是确定是否PPARGC1A(和PPARGC1B)表达式由共同调制等离子体在人类骨骼肌细胞脂肪酸。方法:人类肌管,在体外分化处理0.5更易与十四烷酸/ l (C14:0)、棕榈酸酯(0)、硬脂酸(C18:0) palmitoleate (C16:1omega7)、油酸(C18:1omega9)或亚油酸酯(C18:2omega6)。PPARGC1A / B信使rna被rt - pcr量化。线粒体活动是由甲瓒的形成。结果:未经处理的细胞表达28-fold比PPARGC1A PPARGC1B信使RNA信使RNA (13.33 + / - -2.86 vs 0.47 + -0.08 / fg /杯子总RNA, n = 5)。PPARGC1A表达提高两到三倍的不饱和脂肪酸(ufa)测试(p < 0.05, n = 5)。相比之下,饱和脂肪酸(美国)没有调节PPARGC1A表达式。此外,亚油酸酯的作用不是削弱了棕榈酸酯。PPARGC1B mRNA表达并不是增加了乌法或美国。 SFAs reduced PPARGC1B expression (p<0.05 for palmitate and stearate, n=5). Notably, linoleate reversed palmitate's repressive effect on PPARGC1B. Myotube mitochondrial activity was increased by all UFAs (p<0.01 each, n=5), but was impaired by the SFA stearate (p<0.001, n=5). CONCLUSIONS/INTERPRETATION: We report here that fatty acids differentially regulated expression of the genes encoding the PGC-1 isoforms. Since these effects were accompanied by significant changes in mitochondrial activity, we suggest that the fatty acid-induced regulation of expression of these genes plays an important role in muscle oxidative metabolism.