大黄酸对肠上皮细胞紧密连接的影响在IgA肾病。

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彭SN,曾庆红HH,傅AX,陈为,朱镕基QX、

大黄酸对肠上皮细胞紧密连接的影响在IgA肾病。

世界杂志。2013年7月14日,19 (26):4137 - 45。doi: 10.3748 / wjg.v19.i26.4137。

PubMed ID
23864776 (在PubMed
]
文摘

目的:探讨大黄酸对肠上皮细胞紧密连接蛋白的影响与IgA肾病大鼠(IgAN)。方法:28女性Sprague-Dawley老鼠被随机分为四组每组(7):控制、IgAN Rhein-treated, Rhein-prevented。牛血清白蛋白、脂多糖和亚兰被用来建立IgA肾病大鼠模型。Rhein-treated集团得到了大黄酸从星期7,直到老鼠牺牲。Rhein-prevented集团得到了大黄酸从星期1。动物被牺牲了10周的最后一天。我们观察到肠上皮细胞紧密连接的变化使用透射电子显微镜和肠上皮细胞紧密连接蛋白的表达带occludens蛋白免疫荧光(佐薇)1和occludin的使用激光共焦显微镜。ZO-1 mRNA和蛋白表达的变化和occludin的逆转录酶聚合酶链反应和免疫印迹。尿乳果糖/甘露醇的比率是衡量高效液相色谱(HPLC)对评估肠道通透性。结果:在对照组,上皮细胞之间的紧密连接撒谎的顶端外一侧的细胞膜,并出现在密集的虚线晶体结构,相邻细胞紧密绑定没有显著差距,和紧密连接蛋白ZO-1 occludin均匀分布在肠道上皮细胞的顶部结。 Compared with the control group, in the IgAN group, the structure of the tight junction became obscured and the dotted crystal structures had disappeared; the fluorescence of ZO-1 and occludin was uneven and weaker (5.37 +/- 1.27 vs 10.03 +/- 1.96, P < 0.01; 4.23 +/- 0.85 vs 12.35 +/- 4.17, P < 0.01); the mRNA expression of ZO-1 and occludin decreased (0.42 +/- 0.19 vs 0.92 +/- 0.24, P < 0.01; 0.40 +/- 0.15 vs 0.97 +/- 0.25, P < 0.01); protein expression of ZO-1 and occludin was decreased (0.85 +/- 0.12 vs 1.98 +/- 0.43, P < 0.01; 0.72 +/- 0.15 vs 1.38 +/- 0.31, P < 0.01); and the ratio of urinary lactulose/mannitol increased (3.55 +/- 0.68 vs 2.72 +/- 0.21, P < 0.01). In the Rhein-prevented and Rhein-treated groups, compared with the IgAN group, the intestinal epithelial tight junctions were repaired; fluorescence of ZO-1 and occludin was stronger (11.16 +/- 3.52 and 8.81 +/- 2.30 vs 5.37 +/- 1.27, P < 0.01; 10.97 +/- 3.40 and 9.46 +/- 2.40 vs 4.23 +/- 0.85, P < 0.01); mRNA of ZO-1 and occludin increased (0.81 +/- 0.17 and 0.64 +/- 0.16 vs 0.42 +/- 0.19, P < 0.01; 0.82 +/- 0.22 and 0.76 +/- 0.31 vs 0.40 +/- 0.15, P < 0.01); protein expression of ZO-1 and occludin was increased (2.07 +/- 0.41 and 1.57 +/- 0.23 vs 0.85 +/- 0.12, P < 0.01; 1.34 +/- 0.21 and 1.15 +/- 0.17 vs 0.72 +/- 0.15, P < 0.01); and the ratio of urinary lactulose/mannitol decreased (2.83 +/- 0.43 and 2.87 +/- 0.18 vs 3.55 +/- 0.68, P < 0.01). CONCLUSION: Rhein can enhance the expression of ZO-1 and occludin, repair damaged tight junctions, and protect the intestinal barrier.

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