单核细胞反应结核菌素受体在BCG延迟驱动类型超敏反应。

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Strapagiel D, Kasztalska K, Druszczynska M, Kowalewicz-Kulbat M, Vrba, Matusiak, Chmiela M, Rudnicka W

单核细胞反应结核菌素受体在BCG延迟驱动类型超敏反应。

叶形线Histochem Cytobiol。2008; 46 (3): 353 - 9。doi: 10.2478 / v10042 - 008 - 0044 - 1。

PubMed ID

19056540 (在PubMed

]

文摘

结核病仍然是死亡的主要原因是由于细菌病原体。目前唯一的结核病疫苗、卡介苗(BCG)是在最好的情况下,50%的整体保护效果。皮肤测试与纯蛋白衍生物(产后抑郁症)结核分枝杆菌的方法检测BCG-induced细胞介导免疫性,体内。在前面的研究中我们发现,大约60%的年轻志愿者无结核病史,曾受到新生儿卡介苗接种疫苗和复种(s)在学校时代,开发延迟型超敏反应(DTH)结核菌素。其余志愿者持续结核菌素负的。此外,我们发现一个重要的关联BCG驱动开发潜产后抑郁症和多态性的CD14基因C / T(-159)对巨噬细胞受体识别分枝杆菌化合物。它已经表明,CD14基因变异可能发挥作用在外观和持久性的潜孔在BCG接种对象产后抑郁症。为了扩展我们的研究可能CD14在BCG驱动对产后抑郁症DTH反应,我们测量的表达mCD14巨噬细胞,刺激或不与分枝杆菌抗原刺激,sCD14的血清。考虑到CD14的重要性——TLR2和TLR4在巨噬细胞信号相互作用,我们确定TLR2和TLR4基因的多态性以及巨噬细胞表达TLR2的志愿者与产后抑郁症,没有皮肤反应。我们观察到一个微妙而显著减少附着单核细胞CD14密度从结核菌素阳性与结核菌素-志愿者。 However, we found no difference in CD14 density on monocytes enriched in CD14+ cells using anti-CD14 mAb coupled to magnetic beads. A significant increase in CD14 density was observed on macrophages stimulated with PPD and LPS but not with live BCG bacilli. However, this increase as well as serum levels of soluble sCD14 were similar in the volunteers with and without skin reactions to PPD. Thus, our suggestion on the role of CD14 in the generation of DTH to tuberculin in BCG vaccinated subjects should be further explored. The most important CD14 co-receptors are Toll-like receptors (TLRs) which activate nuclear factors for the production of inflammatory cytokines. However, we could see no association between the polymorphisms of TLR4 (Asp299Gly and Thr399Ile) and TLR2 genes (Arg753Gln and Arg677Trp) and skin responses to PPD. Also, the TLR2 density was similar on monocytes from tuberculin negative and tuberculin positive volunteers.

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药物靶点

药物	目标	类	生物	药理作用	行动
结核菌素纯蛋白衍生物	toll样受体2	蛋白质	人类	是的	配位体	细节