识别氨基酸的c端地区的人类促卵泡激素(FSH) beta-subunit参与人类FSH受体结合。

文章的细节

引用

复制到剪贴板

Lindau-Shepard B,罗斯科,迪亚斯农协

识别氨基酸的c端地区的人类促卵泡激素(FSH) beta-subunit参与人类FSH受体结合。

内分泌学。1994年9月,135 (3):1235 - 40。

PubMed ID

8070368 (在PubMed

]

文摘

最近的分析人类FSH (hFSH)使用antipeptide抗体,单克隆抗体,和hCG的嵌合结构/ hFSH强烈建议c端区域,包括残留81 - 100 hFSH beta-subunit,参与亚基协会以及hFSH异质二聚体结合和/或激活受体。为了测试这个假说,定点诱变用于生成五三丙氨酸突变体的c端地区hFSHβ:Q81, H83, G85;K86、D88 S89;D90 S91, T92;T95 D93, V96;,G98 R97 L99。baculovirus-infected昆虫细胞系统是用于表达。高5细胞感染病毒窝藏δhFSHβ互补DNA(互补)或野生型hFSH贝塔(βhFSH wt)包含hFSHαcDNA互补脱氧核糖核酸和合并感染病毒。感染后,媒体对FSH化验使用heterodimer-specific捕获酶联免疫吸附试验。所有δhFSHβ亚基形成与hFSHαwt亚基形成和分泌的媒介。 These results suggest, for all five mutants, that side chains of amino acids substituted with alanine had no significant role in subunit association. The FSHs delta hFSH and hFSHwt were tested in a RRA, using cell lines that express the hFSH receptor, to determine if there were any changes in binding activity. Similarly, delta hFSH and hFSHwt were compared for receptor activation by measuring the levels of progesterone production in an in vitro FSH bioassay. delta hFSH-(93-96) exhibited minimal binding activity and no detectable steroidogenic activity. delta hFSH-(97-99) showed reduced binding affinity compared with that of hFSHwt, whereas the binding potency and bioactivity of the remaining delta hFSH were comparable to those of hFSHwt. These data demonstrate that within the hFSH beta-(81-99) region, FSH receptor-binding sites are contained within the sequence 93-99.

DrugBank数据引用了这篇文章

药物靶点

药物	目标	类	生物	药理作用	行动
Follitropin	促卵泡激素受体	蛋白质	人类	是的	受体激动剂	细节
Urofollitropin	促卵泡激素受体	蛋白质	人类	是的	受体激动剂	细节