描述一个新的金属-β-内酰胺酶基因,bla (ndm - 1)和一种新型红霉素酯酶基因进行独特的遗传结构在肺炎克雷伯菌序列类型14来自印度。
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Toleman勇D,妈,gisk CG,曹HS, Sundman K,李K,沃尔什TR
描述一个新的金属-β-内酰胺酶基因,bla (ndm - 1)和一种新型红霉素酯酶基因进行独特的遗传结构在肺炎克雷伯菌序列类型14来自印度。
Antimicrob代理Chemother。2009年12月,53 (12):5046 - 54。doi: 10.1128 / AAC.00774-09。Epub 2009年9月21日。
- PubMed ID
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19770275 (在PubMed]
- 文摘
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印度裔的瑞典病人前往新德里,印度,获得了克雷白氏肺炎引起的尿路感染菌株类型序列类型14复杂。隔离,肺炎克雷伯菌05 - 506,被证明具有金属-β-内酰胺酶(MBL),但之前所知MBL基因负。库和放大1类整合子基因显示三个resistance-conferring地区;第一个包含bla (CMY-4)两侧ISEcP1和基线轮廓。4.8 kb的第二区域包含一个复杂的第1类整合子基因arr-2磁带,一个新的红霉素酯酶基因;ereC;aadA1;和cmlA7。一个完整的ISCR1元素被证明是qac /南的下游基因。第三个区域包括一个新的MBL基因,指定bla (ndm - 1),两侧的k .肺炎DNA和截断IS26元素在其另一边。 The last two regions lie adjacent to one another, and all three regions are found on a 180-kb region that is easily transferable to recipient strains and that confers resistance to all antibiotics except fluoroquinolones and colistin. NDM-1 shares very little identity with other MBLs, with the most similar MBLs being VIM-1/VIM-2, with which it has only 32.4% identity. As well as possessing unique residues near the active site, NDM-1 also has an additional insert between positions 162 and 166 not present in other MBLs. NDM-1 has a molecular mass of 28 kDa, is monomeric, and can hydrolyze all beta-lactams except aztreonam. Compared to VIM-2, NDM-1 displays tighter binding to most cephalosporins, in particular, cefuroxime, cefotaxime, and cephalothin (cefalotin), and also to the penicillins. NDM-1 does not bind to the carbapenems as tightly as IMP-1 or VIM-2 and turns over the carbapenems at a rate similar to that of VIM-2. In addition to K. pneumoniae 05-506, bla(NDM-1) was found on a 140-kb plasmid in an Escherichia coli strain isolated from the patient's feces, inferring the possibility of in vivo conjugation. The broad resistance carried on these plasmids is a further worrying development for India, which already has high levels of antibiotic resistance.