溴化的细胞和分子机制glutaraldehyde-didecyldimethylammonium消毒剂对白色念珠菌。

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林W,邓元D, Z,牛B,陈问

溴化的细胞和分子机制glutaraldehyde-didecyldimethylammonium消毒剂对白色念珠菌。

J: Microbiol。2019年1月,126 (1):102 - 112。doi: 10.1111 / jam.14142。Epub 2018年11月21日。

PubMed ID

30365207 (在PubMed

]

文摘

目的:在我们之前的研究中,我们已经开发出一种新的组合消毒剂,必威国际appglutaraldehyde-didecyldimethylammonium溴化(GD)。正是验证了GD有强烈影响大肠杆菌和金黄色葡萄球菌。在这个工作中,白色念珠菌被选为一个对象,它可以被GD。我们旨在调查GD的细胞和分子机制影响白念珠菌。方法和结果:灭菌实验的结果表明,GD在白念珠菌有效。流式细胞仪和原子吸收光谱法检测白念珠菌的细胞膜损伤。荧光素酶反应和布拉德福德法进行了定量检测ATP含量和蛋白质。透射电子显微镜用于细胞内细胞器形态学观察。为了研究线粒体膜电位的变化,自123年作为一个指标。DNA构象分析是由分子建模和圆二色性。 The results indicated that membrane permeability was increased rapidly owing to GD effect, and the leaked K(+) and Mg(2+) were about 12.1 and 12.4 times those of the control, respectively, at 10 min after GD treatment. Simultaneously, ATP and protein also leaked rapidly out of the cell. Mitochondrial membrane potential was destroyed, succinic dehydrogenase activity was significantly decreased and DNA conformation was changed because of GD action. CONCLUSIONS: Glutaraldehyde-didecyldimethylammonium bromide disinfected C. albicans through distorting cell membrane integrity and permeability, disturbing the intracellular homeostasis by intracellular substances leakage, especially K(+) , Mg(2+) , ATP and protein, causing electrolyte imbalance of mitochondria, changing DNA structure, which finally led to cell death. SIGNIFICANCE AND IMPACT OF THE STUDY: This study focused on the cellular and molecular mechanism of GD as a disinfectant against C. albicans. It is important to provide theoretical support to GD against Candida albicans in practical application.

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药物靶点

药物	目标	类	生物	药理作用	行动
Didecyldimethylammonium	磷脂膜	集团	大肠杆菌(应变K12)	未知的	不可用	细节