积累的有机阴离子在培养大鼠肝细胞胞内囊泡是由微管的multispecific有机阴离子转运蛋白。

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Oude Elferink RP, CT,他Roelofsen H, Middelkoop E, Ottenhoff R,简森Heijn M, PL

积累的有机阴离子在培养大鼠肝细胞胞内囊泡是由微管的multispecific有机阴离子转运蛋白。

肝脏病学。1993年3月,17 (3):434 - 44。

PubMed ID

8444418 (在PubMed

]

文摘

肝细胞内运输有机阴离子和可能参与细胞内囊泡与荧光显微镜进行了研究。为此monochlorobimane nonfluorescent疏水性化合物,容易渗透进入细胞并与谷胱甘肽共轭形成荧光谷胱甘肽bimane,使用。在正常大鼠的模型包括肝脏,谷胱甘肽bimane迅速分泌到胆汁。相反,在我们的研究中突变的TR -大鼠的肝脏,减少100倍的谷胱甘肽bimane分泌到胆汁发生。突变TR -老鼠在微管的一种遗传缺陷multispecific有机阴离子转运蛋白介导的ATP-dependent分泌多种有机阴离子在微管的膜进入胆汁。当培养纯种和TR -肝细胞含有谷胱甘肽bimane、两个细胞胞质强荧光显示。纯种细胞完全失去这个胞质荧光在monochlorobimane-free孵化的媒介,因为谷胱甘肽的分泌bimane。一个清晰的点状的荧光,然而,这是分散在细胞细胞核周围的浓度。在一些细胞水泡荧光也集中在一个小沟。相比之下,TR -细胞失去了胞质荧光更慢,完全缺乏水泡荧光。 Making cells selectively permeable with digitonin directly after loading them with glutathione bimane to remove cytosolic fluorescence again revealed the presence of fluorescent vesicles in Wistar cells and their absence in TR- cells. In Wistar cells vesicular fluorescence could be increased by preincubation with monensin or methylamine, compounds that have been shown to interfere with plasma membrane recycling. In conclusion, these results suggest that apart from secretion over the plasma membrane, the canalicular multispecific organic anion transporter may be involved in accumulation of organic anion in intracellular vesicles. It is hypothesized that this intracellular localization of the canalicular multispecific organic anion transporter is caused by recycling of the transporter between the plasma membrane and intracellular membranes.

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药物转运蛋白

药物	转运体	类	生物	药理作用	行动
吲哚菁绿原酸	微管的multispecific有机阴离子转运体1	蛋白质	人类	没有	底物	细节