组织工程软骨的多孔polycarprolactone——藻酸盐支架:组织工程在泰国的第一份报告。

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Bunaprasert T, Thongmarongsri N, Thanakit V, Ruangvejvorachai P, Buranapraditkul年代,Maneesri年代,Kanokpanont年代

组织工程软骨的多孔polycarprolactone——藻酸盐支架:组织工程在泰国的第一份报告。

J地中海Assoc泰国。2006年9月,89,85:S108-14。

PubMed ID

17718275 (在PubMed

]

文摘

目的:工程师人类软骨与多孔聚已酸内酯(PCL)褐藻酸盐支架。背景:聚已酸内酯(PCL)是一个长期的可降解聚合物具有良好的机械强度。作者捏造PCL为ear形状的支架。海藻酸水凝胶用于种子软骨细胞进入PCL多孔支架由gel-cell播种技术。材料和方法:PCL人类耳廓支架制作像粒子浸出技术。从人类的肋软骨和软骨细胞分离培养。培养的软骨细胞混合1.2%海藻酸和b-FGF (basic-fibroblast生长因子)的浓度5 ng / ml 25 x 10(6)细胞/毫升,然后被播种在多孔PCL脚手架结构。构造是在体外培养1周。然后他们在皮下植入6女裸体小鼠的面(5周)。两个裸体小鼠牺牲了2,3,6个月。 Histological study was done (H&E, Alcian blue, collagen type II). RESULT: Neocartilage was formed in the porous cavity of PCL scaffold. At 2 and 3 months, neocartilage were similar to very young cartilage. At 6 months, they were mature. The delayed maturation until 6 months and the highly vascularization of neocartilage in the early phase was the effect of human b-FGF The growths of neocartilage islands in porous cavity were also observed along with degradation ofPCL inter-porous septum. CONCLUSION: This paper reports the first success of cartilage tissue engineering in Thailand.

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药物

Neocartilage