ADH5基因的克隆和表征人类酒精脱氢酶编码5,甲醛脱氢酶。
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埃登贝里户珥MW, HJ
ADH5基因的克隆和表征人类酒精脱氢酶编码5,甲醛脱氢酶。
基因。1992年11月16日,121(2):305 - 11所示。
- PubMed ID
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1446828 (在PubMed]
- 文摘
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人类chi-alcohol脱氢酶(chi-ADH)是一个zinc-containing二聚的酶负责长链醇的氧化和omega-hydroxyfatty酸。第三类抗利尿激素,其中chi-ADH原型,广泛生产和守恒在进化过程中。这表明他们完成重要的管家的角色在细胞的新陈代谢。最近的证据表明,第三类ADH和甲醛脱氢酶(外籍)是相同的酶。我们已经孤立和特征两个重叠的基因克隆,覆盖整个ADH5(外籍)基因。ADH5由9个外显子和八个内含子。两个主要的转录起始点确定引物的扩展。5 ' nontranslated地区不同寻常之处在于,它包含两个额外的上游ATG基码,将编码20和10个氨基酸的肽。无论是上游的ATG在翻译起始的好环境,而ATG启动&khgr;抗利尿激素在一个有利的环境。5 '地区ADH5 CpG岛; it is extremely G+C rich and has many CpG doublets. It does not contain either a TATA box or a CAAT box. This is consistent with ubiquitous expression, and contrasts with the promoters of all previously cloned ADH genes, which are expressed in a tissue-specific manner. The 5' region of ADH5 contains consensus binding sites for the transcriptional regulatory proteins, Sp1, AP2, LF-A1, NF-1, NF-A2, and NF-E1. A 1.5-kb upstream fragment from ADH5 was able to drive the transcription of a cat reporter gene at high levels in monkey kidney cells (CV-1). Several processed pseudogenes were also isolated.